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在SARS-CoV-2感染时内源逆转录病毒的识别和特征

Xuefei Guo¹, Yang Zhao¹, Fuping You¹

¹Institute of Systems Biomedicine, Department of Immunology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, National Health Commission (NHC) Key Laboratory of Medical Immunology, Peking University Health Science Center, Beijing, China.

Frontiers in immunology

|April 22, 2024

概括

内源逆转录病毒 (ERVs) 在COVID-19患者和动物模型中受到上调,这表明它在疾病发病过程中发挥了作用. 特定的ERV可以作为COVID-19严重程度的生物标志物.

科学领域:

基因组学就是基因组学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

内源逆转录病毒 (ERV) 是古代病毒感染的遗留物,占人类基因组的8%左右.
在COVID-19病变发生过程中ERVs的作用尚不清楚.
ERVs来自长终端重复 (LTR) 转位子家族.

研究的目的:

为了研究ERVs在对SARS-CoV-2感染的反应中的全基因组表达.
为了确定COVID-19严重程度的潜在ERV生物标志物.
探索与上调ERV相关的生物过程.

主要方法:

在人类外周血液单核细胞 (hPBMC),子和小鼠细胞中全基因组识别ERV.
对感染者与未感染者的ERV表达的分析.
使用基因组区域丰富注释工具 (GREAT) 进行基因组序列分析.
在小鼠模型中进行RT-qPCR验证.

主要成果:

在hPBMCs,子和小鼠中鉴定出明显上调的可移植元素 (TE) 和ERVs.SARS-CoV-2感染后.
在严重的COVID-19病例中发现了210个专门上调的ERV,这些ERV与免疫反应和基因素修饰有关.
HERV1_I-int:ERV1:LTR和LTR7Y:ERV1:LTR被确定为潜在的严重性生物标志物.
父母基因组序列上调的ERVs涉及质子修饰和病毒复制.

结论:

在病毒感染后,ERV表达模式发生变化,包括SARS-CoV-2.
上调的ERV,特别是在严重的COVID-19中,与免疫激活和表观遗传修饰有关.
特定的ERV显示为评估COVID-19严重程度的生物标志物具有前途.
这项研究为了解ERV-SARS-CoV-2相互作用提供了基础.

关键词:

在 COVID-19 疫情中,在ERV系统中,在此期间,Erv1 在RNA-Seqq.这就是SARS-CoV-2病毒.这就是为什么特E是TEs.

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