生物合成和代谢途径控制了有氧异构体中的氨基酸H值
Shaelyn N Silverman1, Reto S Wijker1, Alex L Sessions1
1Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, United States.
Frontiers in microbiology
|April 22, 2024
概括
氨基酸中的同位素比例揭示了对微生物代谢的洞察力. 这些δ2H值反映了生物合成和代谢途径,为环境生理学研究提供了潜力.
科学领域:
- 生物地质化学生物地质化学
- 微生物生理学 微生物生理学
- 稳定的同位素生态学
背景情况:
- 氨基酸 (AA) 中的同位素比率 (δ2H) 在各个生物和组织中存在显著差异.
- 这些变异表明丰富的生化信息,但基本的控制是不太了解.
- 虽然脂质中的δ2H与NADPH代谢有关,但氨基酸控制仍然难以捉摸.
研究的目的:
- 为了研究氨基酸中的δ2H值的生化控制.
- 确定生物合成和代谢途径如何影响氨基酸 δ2H.
- 评估氨基酸δ2H作为微生物生理学代理物的潜力.
主要方法:
- 在五种在不同碳基板上生长的有氧异质微生物中测量了氨基酸的δ2H值.
- 分析了五种与改变NADPH代谢的埃舍里希亚大肠杆菌突变菌株中的δ2H.
- 与已知的生物合成和代谢途径相关的 δ2HAA 模式.
主要成果:
- 在不同的生物体和生长条件中观察到一致的 δ2HAA 模式.
- 证明生物合成途径是δ2HAA的主要控制.
- 显示 δ2HAA 值会随着代谢途径和关键代谢物如酸盐和NADPH而有系统的变化.
结论:
- 氨基酸 δ2H 值受到细胞代谢,特别是生物合成和代谢的强烈影响.
- 这些同位素信号由包括NADPH在内的关键代谢物调节.
- 氨基酸 δ2H 对调查环境生物体生理学和新陈代谢具有前景.
更多相关视频
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
9.4K
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
4.8K
相关概念视频
What is Metabolism?
114.0K
Overview
114.0K
Overview of Nitrogen Metabolism
7.9K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.9K
Overview of Protein Metabolism
1.1K
Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
1.1K
Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Overview of Fatty Acid Metabolism
30.6K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
30.6K
Overview of Metabolism
30.1K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
30.1K
