在内皮真空膜中丰富的水素蛋白调节高糖血症中微血管的直径
Changsheng Chen1, Yinyin Qin2,3, Yidan Xu4,5
1School of Life Sciences, Nantong Laboratory of Development and Diseases, Nantong University, Nantong, China.
Cardiovascular research
|April 22, 2024
概括
内皮细胞 (ECs) 中的水蛋白通过细胞内真空管调节血管直径,为糖尿病血管并发症提供潜在的基因治疗点. 这项研究揭示了治疗糖尿病血液供应不足的新机制.
科学领域:
- 血管生物学 血管生物学
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
背景情况:
- 糖尿病微血管并发症导致血液供应减少,原因是血管直径下降.
- 这些血管缺陷的调节机制和有效治疗方法尚不清楚.
研究的目的:
- 为了研究水利素在糖尿病血管并发症中的作用.
- 确定潜在的治疗点,以改善糖尿病患者的血液 perfusion.
主要方法:
- 在糖尿病视网膜样本和高葡萄糖处理的内皮细胞 (ECs) 中评估人体水素1 (AQP1) 表达.
- 利用斑马鱼胚胎研究高葡萄糖对血管直径和 perfusion 的影响.
- 使用功能丧失和过度表达模型研究斑马鱼水素素 (aqp1a.1和aqp8a.1) 的功能.
- 研究了水素素在细胞内真空和血管发光中的局部化和作用.
主要成果:
- 在糖尿病患者的人类视网膜和高葡萄糖治疗的EC中观察到AQP1表达的减少.
- 高葡萄糖降低了斑马鱼胚胎的血管直径和输液,与aqp1a.1和aqp8a.1的下调有关.
- 失去了aqp1a.1/aqp8a.1模仿高血糖血管缺陷;他们的过度表达拯救了这些缺陷和扩大的血管.
- 水氨酸位于细胞内真空中,对于EC光化至关重要;它们的损失损害了血管形成.
结论:
- 内皮细胞特异性水素调节血管直径通过细胞内真空介导的过程在高血糖条件下.
- 在EC中的水素素是基因疗法治疗糖尿病血管输液缺陷的有希望的标.
更多相关视频
10:39Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo
Published on: March 8, 2018
8.3K
10:07Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tgfli:EGFP Zebrafish Retinal Vasculature
Published on: December 26, 2017
13.2K
相关概念视频
Arteries and Arterioles
3.6K
Arteries, the vasculature responsible for transporting blood from the heart, possess robust walls capable of enduring the elevated pressures exerted by the heartbeat. Arteries near the heart are especially thick-walled and enriched with elastic fibers across their three tunics, classifying them as elastic or conducting arteries. These arteries, usually with a diameter exceeding 10 mm, are characterized by their ability to dilate in response to the blood pumped from the heart's ventricles...
3.6K
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Glomerular Filtration Rate and its Regulation
2.6K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
2.6K
Hormones Regulating Blood Glucose
3.3K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
3.3K
Autoregulation of Blood Flow
2.3K
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
2.3K
Endocrine Signaling
64.3K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
64.3K
