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Cell Migration01:19

Cell Migration

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Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Cytoskeletal Coordination in Cell Migration01:32

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Gastrulation01:56

Gastrulation

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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相关实验视频

Updated: Jun 28, 2025

Modeling and Imaging 3-Dimensional Collective Cell Invasion
07:08

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Published on: December 7, 2011

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通过细胞分裂架起桥梁的两个顺序基因表达程序支持长距离的集体细胞迁移.

Jingjing Sun1, Ayse Damla Durmaz1,2, Aswini Babu1

  • 1California Institute of Technology, Division of Biology and Biological Engineering, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Development (Cambridge, England)
|April 22, 2024
PubMed
概括

在Drosophila胚胎中的基因表达程序指导细胞迁移. 通过细胞分裂连接的两个连续程序,在中肠发育过程中控制尾部内脏半皮体 (CVM) 细胞运动.

关键词:
尾巴内脏半皮体中的尾巴内脏半皮体细胞周期进展的进展集体细胞迁移 集体细胞迁移时间空间基因表达.转录程序 转录程序 转录程序

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科学领域:

  • 发育生物学是发展生物学.
  • 细胞生物学 细胞生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 精确的组织和器官组装需要时空基因表达调节.
  • 协调集体细胞行为对于发育至关重要.
  • 了解细胞迁移期间的基因表达动态是必不可少的.

研究的目的:

  • 为了研究在Drosophila胚胎中迁移期间尾部内脏半皮 (CVM) 细胞的基因表达变化.
  • 为了确定控制CVM迁移期间基因表达的cis-regulatory元素.
  • 在集体细胞迁移过程中阐明基因表达程序的潜在机制.

主要方法:

  • 对10个CVM表达基因及其cis调节序列的分析.
  • 研究细胞循环进展在调节基因表达中的作用.
  • 使用基因操纵研究转录因子功能 (例如,E2F1,牛,Zfh1,Dorsocross).

主要成果:

  • 在CVM细胞迁移过程中确定了早期和晚期的基因表达程序.
  • 证明受字符串/Cdc25影响的细胞周期进展加速过渡到晚期基因表达.
  • 发现E2F1是晚期基因CG5080.0的关键转录因子.
  • 显示的早期基因转录是前后两极分化的,取决于Snail,Zfh1和Dorsocross.

结论:

  • 两个不同的,顺序的基因表达程序协调CVM细胞迁移.
  • 细胞分裂作为早期和晚期基因表达阶段之间的桥梁.
  • 特定的转录因子调节集体迁移期间基因表达的空间和时间控制.