在Trichoderma koningii中通过固体基质发酵生产,净化和确定β-葡萄糖酶的生物化学特性
Selma Çelen Yücetürk1, Ayşe Dilek Azaz2
1Department of Molecular Biology and Genetics, Faculty of Science and Literature, 53003 Balikesir University , 10145 Balıkesir, Türkiye.
Zeitschrift fur Naturforschung. C, Journal of biosciences
|April 22, 2024
概括
这项研究通过固体基质发酵从Trichoderma koningii中净化β-葡萄糖酶,使用玉米. 该酶在pH值3.0和75°C时表现出高活性,并受到葡萄糖和葡萄糖酸盐的竞争性抑制.
科学领域:
- 酶学 是一种酶学.
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
背景情况:
- β-葡萄糖酶对于纤维素水解至关重要.
- 特里科德玛koningii是众所周知的工业酶的来源.
- 固体基质发酵 (SSF) 提供了一种有效的酶生产方法.
研究的目的:
- 从Trichoderma koningii中分离和净化β-葡萄糖酶.
- 描述纯化酶的生化和运动性质.
- 为了优化酶的生产使用SSF与玉米.
主要方法:
- 在玉米上进行Trichoderma koningii的固体基质发酵 (SSF).
- 两步净化:硫酸沉和疏水相互作用色谱.
- 生物化学和动力学表征,包括pH,温度,Km和Vmax的确定.
- 用SDS-PAGE和NATIVE-PAGE进行评估的酶纯度.
主要成果:
- 在30°C下6天获得最佳的酶生成.
- 净化产生了22.56倍的增长,效率为73.51%.
- 在pH值3.0和75°C时观察到最佳活性.
- 分别确定Km和Vmax值为0.16 mM和2000 EU.
- 通过d-(+) - 葡萄糖和 δ-葡萄糖酸进行竞争性抑制.
结论:
- 从T. koningii中高效地生产和净化β-葡萄糖酶,使用SSF.
- 描述的酶表现出有利于工业应用的特性.
- 了解酶动力学和抑制可以为过程优化提供洞察力.
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