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测试和优化iDNA的metabarcoding从甲虫的样本哺乳动物在超多样化的新热带地区的样本

Bruno H Saranholi^1,2, Filipe M França^3,4, Alfried P Vogler^1,5

¹Department of Life Sciences, Imperial College London, Ascot, UK.

Molecular ecology resources

|April 22, 2024

概括

泥甲虫有效地使用摄入的DNA (iDNA) 来样本哺乳动物的多样性. 16SrRNA迷你条形码恢复了比12SrRNA更多的哺乳动物物种,改进了热带哺乳动物社区评估iDNA方法.

科学领域:

生态生态学生态生态学
分子生态学分子生态学
保护生物学保护生物学

背景情况:

昆虫,特别是便甲虫,越来越多地被用作通过摄入DNA (iDNA) 分析来检测脊椎动物多样性的样本.
甲甲虫是适合的哺乳动物采样者,因为它们的饮食多样化,分布广泛,并且很容易收集.

研究的目的:

优化使用来自虫的iDNA来评估哺乳动物群落.
评估生物和方法因素如何影响便甲虫作为哺乳动物样本.

主要方法:

使用60个陷,收集了403只甲甲虫.
通过metabarcoding对每个甲虫的摄入DNA (iDNA) 进行了测序,使用12SrRNA和16SrRNA迷你条形码.
评估了甲甲虫特征 (食,筑巢,大小) 和杀死解决方案对哺乳动物iDNA恢复的影响.

主要成果:

确定了50个哺乳动物操作分类单位 (OTU),包括来自10个类的陆地和树木物种.
从70%的甲甲虫标本中获得哺乳动物DNA序列.
16SrRNA恢复了比12SrRNA更多的哺乳动物OTU,但这两种标记物都显示了部分重叠,表明需要用于综合多样性评估的联合使用. 泥甲虫的特征和杀死解决方案并没有显著影响检测到的哺乳动物OTU的数量.

结论:

这项研究改进了使用虫iDNA进行热带哺乳动物群体常规评估的方法.
这些发现支持了该方法的普遍适用性,不管是当地虫物种特征还是使用的杀死解决方案,尽管建议使用12SrRNA和16SrRNA标记器来最大限度地检测哺乳动物多样性.

关键词:

亚马逊雨林是亚马逊雨林中的一个.生物多样性生物多样性生物监测生物监测不脊椎动物衍生的DNA.超级编码元标段编码.

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