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Updated: Jun 28, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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序列动机的大规模并行识别触发了与核糖体相关的mRNA质量控制

Katharine Y Chen1,2, Heungwon Park1, Arvind Rasi Subramaniam1

  • 1Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.

Nucleic acids research
|April 22, 2024
PubMed
概括

研究人员通过分析酵母中的子对来确定了新的mRNA衰变触发器. 某些二重复,如带有充电残留的二重复,通过减缓核糖体和激活质量控制途径来破坏mRNA的稳定性.

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Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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