植物衍生的人造miRNA通过喷雾诱导的基因沉默有效地减少了虫 (Aphidoidea) 的增殖
Yuan Wang1, Xuanlin Li1, Chenghong Zhu2
1State Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China.
Pest management science
|April 22, 2024
概括
来自植物的小RNAs (sRNAs) 显示为控制虫的环保生物杀虫剂具有前途. 通过喷雾诱导基因沉默 (SIGS) 应用的人造微RNA (amiRNA) 为化学农药提供了稳定有效的替代品.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 虫对全球农业构成重大威胁,目前的控制严重依赖化学农药.
- 迫切需要经济和环保的害虫管理策略.
- 喷雾诱导基因沉默 (SIGS) 是一种有前途的RNA干扰 (RNAi) 方法,但双链RNA (dsRNA) 稳定性和成本存在局限性.
研究的目的:
- 研究小RNAs (sRNAs) 的潜力,特别是microRNAs (miRNAs),作为SIGS中用于虫管理的调节剂.
- 评估植物衍生人工微RNA (amiRNA) 作为可持续生物杀虫剂的有效性.
- 为了比较amiRNAs与化学合成RNA调节者的有效性.
主要方法:
- 精选的sRNA序列 (miR-9b和miR-VgR) 针对重要的虫基因 (ABCG4和VgR).
- 在实验室条件下通过喷雾处理使用化学合成的单链RNA (syn-ssRNA) 验证了疗效.
- 生成的转基因植物表达人工miR-9b (amiR-9b) 和miR-VgR (amiR-VgR),用于植物衍生的amiRNA生产.
主要成果:
- 喷雾应用syn-ssRNA导致虫存活率降低和异常率增加.
- 来自植物的amiRNAs在明显低于化学合成物质的度下显示出强大且持久的抑制功效.
- 同时应用amiR-9b和amiR-VgR显示了增强的抑制作用.
结论:
- 来自植物的amiRNAs是基于SIGS的虫害管理的有希望和稳定的解决方案.
- 这种方法为现场应用提供了一种持久且可能更经济的替代传统化学农药.
- 在害虫管理策略中进一步优化amiRNA的部署是有必要的.
相关概念视频
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:50Transgenic Plants
7.2K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K