运营条件对饮用水生物膜动态和大肠杆菌入侵潜力的影响
Fien Waegenaar1,2, Cristina García-Timermans1,2, Josefien Van Landuyt1,2
1Department of Biotechnology, Center for Microbial Ecology and Technology (CMET), Ghent University, Ghent, Belgium.
Applied and environmental microbiology
|April 22, 2024
概括
饮用水中的生物膜可能含有大肠杆菌,如Serratia fonticola,构成公共卫生风险. 了解生物膜入侵和脱离对于保持配送系统的安全饮用水质量至关重要.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 公共卫生 公共卫生
背景情况:
- 饮用水生物膜是微生物息地,可以降低水质,并容纳微生物.
- 虫被用作水污染和消毒效率的指标.
研究的目的:
- 调查成熟饮用水生物膜对大肠杆菌入侵的易感性.
- 描述地表水和地下水来源的生物膜之间的差异.
- 评估生物膜对微小操作变化的弹性.
主要方法:
- 使用了带有玻璃环的生物膜监视器,用于生物膜生长和采样.
- 采用流细胞计,ATP测量,共聚焦激光扫描显微镜和16SrRNA测序进行表征.
- 通过改变pH值和自由水平来模拟操作变化.
主要成果:
- 从经过处理的地下水中获得的生物膜的细胞密度高于表面水中的生物膜.
- 在两种生物膜类型之间观察到显著的表型和基因型差异.
- 成熟的生物膜成功地被低度的大肠杆菌Serratia fonticola侵入.
- 这两种生物膜都对模拟的操作变化表现出了弹性.
结论:
- 成熟的饮用水生物膜容易受到大肠杆菌的侵袭,这可能会引起公众健康的担忧.
- 生物膜的特性根据水源 (地表水与地下水) 不同.
- 对生物膜脱落和病原体持久性的进一步研究对于确保饮用水安全至关重要.
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