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在光学清除的大脑中揭示了MRI对比度

Shimrit Oz¹, Galit Saar², Shunit Olszakier¹

¹Department of Neuroscience, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, 3525433, Israel.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|April 22, 2024

概括

光学清除的组织可以与磁共振成像 (MRI) 在补水后兼容,挑战了脂质对于MRI对比至关重要的概念. 这项工作使得多式核磁共振-光显微镜成像成为可能.

科学领域:

神经成像是一种神经成像.
生物医学工程生物医学工程
显微镜的使用方法

背景情况:

光学清除的组织通常被认为不适合磁共振成像 (MRI) 由于缺乏对比度.
以前的研究将MRI对比度与脂质含量联系在一起,阻碍了多式成像方法.
脂质的作用和非脱脂清除方法与MRI的兼容性仍然不清楚.

研究的目的:

通过使用各种组织清除技术,研究光学清除全脑MRI对比度和脂质含量之间的关系.
为了确定MRI对比是否可以在没有脂质的清除组织中恢复.
开发一种多模式的MRI-光显微镜 (LM) 成像方法.

主要方法:

用DDISCO,ECi,Scale和CLARITY方法清除的整个大脑中的MRI对比度的评估.
评估组织补水对MRI兼容性的影响.
组织扩张,水分水平和MRI对比度之间的相关性分析.
开发一个多模式的MRI-LM成像管道.

主要成果:

uDISCO和ECi清除的大脑在补水后是MRI兼容的,尽管有显著的脂质去除.
清除度的大脑,保存脂质,缺乏MRI对比度.
在没有脂质的CLARITY清除的大脑中,MRI对比度在没有脂质重新引入的情况下成功恢复.
组织扩张和过水度与MRI对比度损失密切相关.

结论:

脂质对于在清除的组织中实现MRI对比度并不必不可少.
重水是恢复清除标本MRI兼容性的关键.
这项研究为多模式MRI-LM成像奠定了基础,弥合了微和中等尺度分析.

关键词:

磁力共振成像-对比度大脑大脑大脑的大脑大脑脂质脂质脂质是一种多模式成像 - 多模式成像组织清除 - 组织清除.

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