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在蒸汽预处理过程中添加硫酸盐增强了酶介导的纤维素水解和乙醇生产
Na Zhong1,2, Richard Chandra3, Minna Yamamoto2
1Department of Wood Science, Faculty of Forestry, Forest Products Biotechnology and Bioenergy Group, The University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada.
Bioresources and bioprocessing
|April 22, 2024
概括
在软木的蒸汽预处理过程中添加酸硫化物可以改善生物质加工. 这种方法增强了酶性水解和发酵,从而从基纤维素材料有效生产乙醇.
科学领域:
- 生物质预处理 生物质预处理
- 生物化学工程 生物化学工程
- 可持续化学 可持续化学
背景情况:
- 在蒸汽预处理期间的宁凝结阻碍了酶性水解和发酵.
- 优化预处理条件对于高效的纤维素生物燃料生产至关重要.
- 硫酸盐的添加正在探索,以减轻木质素的凝结,并提高生物质的消化能力.
研究的目的:
- 评估在不同条件下 (酸性,中性,性) 添加硫酸盐对软木的蒸汽预处理的影响.
- 尽量减少木质素的凝结,提高生物燃料生产的整体效率.
- 评估预处理生物质的随后的酶化水解和发酵.
主要方法:
- 在酸性,中性和性条件下用硫酸盐添加软木的蒸汽预处理.
- 对红素凝结和颗粒大小减小的分析.
- 预处理基质的酶性水解.
- 水解酸的发酵以产生乙醇.
主要成果:
- 在210°C以酸硫酸盐蒸汽处理10分钟,产生均的颗粒大小减少.
- 不溶于水的成分由62%的碳水化合物和33%的素组成.
- 高固体负荷 (25% w/v) 实现了58%的水解产量和高效的发酵 (46.6 g/L乙醇).
结论:
- 在软木的蒸汽预处理过程中添加酸硫酸盐,有效地减少了木质素的凝结.
- 这种预处理策略提高了酶解水解和发酵效率.
- 该工艺适用于高固体负载,从而从基纤维素生物质中获得高效的乙醇产量.
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