使用Saccharomyces cerevisiae进行环保发酵,以增加肉的生物活性
Osama M Darwesh1, Aya S Eweys2,3, Yan-Sheng Zhao3
1Agricultural Microbiology Department, National Research Centre, Cairo, 12622, Egypt. darweshosama@yahoo.com.
Bioresources and bioprocessing
|April 22, 2024
概括
用Saccharomyces cerevisiae发酵肉显著提高了其抗氧化和抗炎性质. 这一过程增强了有益的化合物,使发酵肉成为功能性食品的有希望的成分.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 肉 (Cinnamomum cassia) 以其药用特性而闻名.
- 发酵是一种可以改变食品成分生物化学成分和生物活性的过程.
- 细菌酵母是一种广泛用于发酵的酵母.
研究的目的:
- 研究Saccharomyces cerevisiae发酵对肉生物特性的影响.
- 确定发酵的最佳条件,以最大限度地提高抗氧化剂产量.
- 评估发酵肉抗氧化,抗微生物,抗炎和抗癌活性的变化.
主要方法:
- 肉是在各种条件下使用Saccharomyces cerevisiae发酵的.
- 确定了最佳的发酵参数 (酵母度,水量,pH,温度,化时间).
- 使用ABTS,DPPH和H2O2激素清除试验来评估抗氧化活性.
- 总的和黄酸盐含量被量化.
- 对特定的细菌和真菌菌株进行了抗菌活性测试.
- 对抗炎症性质进行了评估.
- 评估了对Huh7细胞的抗癌作用.
- 高性能液体染色学 (HPLC) 用于分析关键酸的变化.
主要成果:
- 最佳发酵发生在10^7 CFU的S. cerevisiae/10克肉,70毫升dH2O,pH 6,在35°C进行3天.
- 发酵的肉显示出显著增加的抗氧化活性 (ABTS,DPPH,H2O2清理43.8-71.9%).
- 总的和黄分别增加了81.3%和415%.
- 发酵的肉对L. monocytogenes,S. aureus,E. coli,S. typhi和C. albicans表现出抗菌活性.
- 抗炎性质从89%提高到92%.
- 淋化提取物表现出抗癌作用,在700μg/mL下降了31%的Huh7细胞活力.
- 在HPLC分析中,p-基酸,甘酸,甲基素,基酸,咖啡酸和酸的含量增加.
结论:
- 糖菌的发酵是一种有效的方法来增强肉的生物特性.
- 发酵的肉表现出改善的抗氧化,抗微生物,抗炎和抗癌活性.
- 这个过程提供了一个可行的策略,用于开发功能性食品,增加健康益处.
更多相关视频
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
11.2K
07:38Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
42.0K
相关概念视频
Microbial Fermentation
1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Microbes in Food Production
407
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
407
Microbes in Beverage Production
300
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
300
Microbes in the Production of Fermented Foods
330
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
330
Bioreactor Controls-III
70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
