氧化在Shiraia菌体培养中调解红光诱导的烯生产
Wen Juan Wang1, Xin Ping Li1, Wen Hao Shen1
1College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.
Bioresources and bioprocessing
|April 22, 2024
概括
红灯通过诱导氧化 (NO) 信号来提高Shiraia真菌中的烯 (PQ) 生产. 这一发现提供了一种有希望的方法来增加这些有价值的光敏剂的产量,用于光动力学疗法.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生化学
- 摄影生物学 摄影生物学
背景情况:
- 来自Shiraia真菌的烯基 (PQs) 是用于光动力学治疗的强有力的光敏化剂.
- 在菌体培养中PQ的低产量阻碍了临床应用.
- 光是已知的真菌二次代谢物生物合成的调节者.
研究的目的:
- 调查氧化 (NO) 在调节Shiraia sp.生长和PQ生物合成中的作用. 在红灯下S9.
- 阐明红光中介PQ生产所涉及的信号通路.
主要方法:
- 对Shiraia sp.进行持续的红光照射. 在S9菌培养物中.
- 测量NO生成,化,枝分支和PQ积累的情况.
- 无氧化物供体 (SNP),无氧化物清除剂 (carboxy-PTIO) 和信号抑制剂 (NS-2028) 的应用.
- 细胞内Ca2+和细胞外ATP (eATP) 水平的分析.
- 使用HPLC测量低素A (HA) 的量化.
主要成果:
- 红光 (627nm) 抑制了结合,促进了分支,并显著增加了PQ积累.
- 红光诱导通过氧化合成酶 (NOS) 或酸盐减少酶 (NR) 路径产生NO.
- 化 (SNP) 处理恢复了化和增强了PQ生产,这取决于NO信号.
- 红光暴露增加了细胞内Ca2+和细胞外ATP (eATP),这与NO生成相互依赖.
- 结合红光和SNP治疗的结果是低素A (254 mg/L) 增加了3.0倍.
结论:
- 红光诱导的NO作为一个信号分子,调节Shiraia sp.的生长和PQ生产. 通过NO-cGMP-PKG路径通过S9.
- NO,Ca2+和eATP之间的合作增强了膜透性和PQ基因表达.
- 结合红光和SNP治疗是一种有前途的策略,可以显著提高真菌中的PQ产量.
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