血中来自大脑的外体环RNAs作为急性缺血性中风的诊断生物标志物
Xinli Jiang1,2, Rui Zhang1,3, Geng Lu3
1Department of Emergency Medicine, Nanjing Drum Tower Hospital, Nanjing Drum Tower Hospital Clinical College of Nanjing University Of Chinese Medicine, Nanjing, Jiangsu, 210008, China.
概括
需要用于急性缺血性中风 (AIS) 诊断的新生物标志物. 研究人员在等离子体外体中发现了特定的circRNAs,这些circRNA显示出早期AIS检测和预后评估的前景.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 急性缺血性中风 (AIS) 是导致死亡和残疾的主要原因.
- 早期诊断AIS对于有效治疗和改善患者结果至关重要.
- 细胞外囊泡,特别是外体和它们所含的circRNA,正在成为潜在的疾病生物标志物.
研究的目的:
- 为了研究血大脑衍生外体中circRNAs作为AIS的生物标志物的潜力.
- 为了确定与健康对照组相比,在AIS患者中表达差异的特定circRNAs.
主要方法:
- 高通量测序用于识别AIS患者的血大脑衍生外体中的失调的circRNAs.
- 实时定量逆转录PCR (qRT‒PCR) 用于在更大的队列中验证.
- 用临床尺度进行相关性分析,例如国家卫生研究院中风量表 (NISS) 和修改的兰金度表 (mRS).
主要成果:
- 确定了358个调节失调的circRNAs,其中23个是上调的,335个是下调的.
- 五个特定的上调circRNAs (hsa_circ_0007290,hsa_circ_0049637,hsa_circ_0000607,hsa_circ_0004808和hsa_circ_0000097) 得到了验证. 在这些研究中,我们发现了
- 这些经过验证的circRNAs在AIS检测方面表现出高的特异性和灵敏性.
- 这些circRNAs的表达水平与AIS严重程度 (NISS和mRS得分) 有正相关.
结论:
- 血大脑衍生外体中的circRNAs代表了对AIS早期诊断的有希望的非侵入性生物标志物.
- 这些circRNAs也可以作为AIS进展和严重程度的指标.
- 对这些外体环RNA的进一步研究可能会为AIS带来更好的诊断和预后工具.
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