通过Mycolicibacterium neoaurum菌株从植物中生物制造丸激素:"一",两种模式
Daria N Tekucheva1, Vera M Nikolayeva2, Mikhail V Karpov2
1G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Center for Biological Research of the Russian Academy of Sciences", Prospect Nauki 5, Pushchino, Moscow Region, 142290, Russia. tekuchevadn@gmail.com.
Bioresources and bioprocessing
|April 22, 2024
概括
这项研究表明,使用Mycolicibacterium neoaurum菌株从植物中有效地产生一性丸激素. 优化的条件产生了创纪录的4.59g/L丸激素,突出了一个具有成本效益的生物生产方法.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- ,主要的男性激素,对于各种生理功能至关重要.
- 目前的激素生产方法可能是复杂和昂贵的.
- 植物提供了一个易于获得和廉价的前体,用于类固醇合成.
研究的目的:
- 开发一种高效的单生物生产方法,从植物中生产丸激素.
- 使用特定微生物菌株优化生物转化过程.
- 为了实现高的产量,绕过中间化合物隔离.
主要方法:
- 使用了Mycolicibacterium neoaurum VKM Ac-1815D和Ac-1816D菌株用于植物的生物转化.
- 在单个生物反应器中采用了两阶段的过程:氧化转化为17-ketoandrostanes和还原转化为.
- 优化条件,包括有氧和微气友环境,以及减少阶段的葡萄糖补充.
主要成果:
- 这两种M. neoaurum菌株都成功地将植物醇转化为.
- 据报道,使用M. neoaurum VKM Ac-1815D从10 g/L的植物中获得了4.59 g/L (摩尔产量>66%) 的最高丸激素产量.
- 优化微气友条件和葡萄糖补充剂显著提高了的产量.
结论:
- 菌株Mycolicibacterium neoaurum是有效的直接生物生产的丸激素从植物醇.
- 开发的单方法为具有成本效益的激素合成提供了显著的进步.
- 这项研究为植物的生物转化途径和微生物类固醇生产提供了宝贵的见解.
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