通过Bacillus natto通过化学调节器进行适应性进化来增强menaquinone-7生物合成
Bei Zhang1, Cheng Peng1, Jianyao Lu1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Bioresources and bioprocessing
|April 22, 2024
概括
使用甘酸盐 (一种化学调节剂) 的适应性进化显著增强了Bacillus subtilis中的menaquinone-7 (MK-7) 生物合成. 这一战略使MK-7的生产提高了2.5倍,为维生素K2的工业制造提供了一种新的方法.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 代谢工程是代谢工程.
背景情况:
- 维生素K2的一种形式menaquinone-7 (MK-7) 对心血管健康,骨密度和预防动脉化至关重要.
- 目前的MK-7生物合成方法需要优化,以便在工业规模生产.
研究的目的:
- 开发一种适应性进化策略,使用化学调节器来增强Bacillus subtilis的MK-7生物合成.
- 为了确定基因机制,提高了MK-7的生产.
主要方法:
- 适应性进化 Bacillus subtilis 使用草甘,一个抑制剂的-5-enolpyruvylshikimate-3-酸盐 (EPSP) 合成酶,作为一个化学调节器.
- 进行比较转录组学分析,以调查增强MK-7生产的遗传基础.
主要成果:
- 与原始菌株相比,调整后的菌株ALE-25-40获得了62 mg/L的最大MK-7标位和0.42 mg/L·h的生产率,分别增加了2.5倍和3倍.
- ALE-25-40表现出减少子形成和增加NADH和氧化还原潜力.
- 转录组分析揭示了与分泌相关的基因的下调和参与NADH形成的基因的上调.
结论:
- 使用草甘的自适应进化是改善Bacillus subtilis.MK-7生物合成的有效策略.
- 增强的性能与基因表达的改变有关,特别是在化和NADH代谢方面.
- 这种方法为维生素K2的工业生产提供了一个有希望的新方向.
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