相关实验视频

Updated: Jun 28, 2025

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

在Gluconobacter oxydans中使用代谢工程和食批量策略进行有效的1,3-二氧化生物合成

Weizhu Zeng^1,2,3,4, Xiaoyu Shan^1,2,3, Li Liu^1,2,4

¹Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

Bioresources and bioprocessing

|April 22, 2024

概括

研究人员通过使用Gluconobacter oxydans增强了1,3-二氧化 (DHA) 的产生. 基因淘汰和媒介优化显著增加了DHA产量,通过食批发发酵达到198.81g L-1.

科学领域:

生物技术是生物技术.
工业微生物学工业微生物学

背景情况:

1,3-二氧 (DHA) 是一种有价值的化学物质,在化品,药品和食品中具有应用.
目前的工业生产依赖于Gluconobacter oxydans,但需要改进工艺以获得更高的产量.

研究的目的:

提高工业生产的1,3-二氧化 (DHA).
确定和实施提高DHA合成效率的策略在Gluconobacter oxydans.

主要方法:

对五种工业野生型Gluconobacter菌株进行选,以选择最佳生产者 (G. oxydans WSH-003).
单独淘汰16个非DHA合成相关的脱酶基因,以确定有益的突变.
优化种子培养和发酵介质组成.
在5L生物反应器中实施料批发发酵系统.

主要成果:

选择的 G. oxydans WSH-003 菌株显示出优异的 DHA 合成.
一种淘汰菌株与野生类型相比,显著增加了42.27%的DHA生产.
优化的介质和料批发发酵导致最终的DHA度为198.81g L-1.1.
达到高的糖转化率为82.84%.

结论:

对G. oxydans的基因改造,特别是通过向基因淘汰,可以显著改善DHA的产生.
媒体优化和先进的发酵策略对于最大限度地提高DHA产量至关重要.
开发的过程为工业DHA合成提供了一种高效的方法.

关键词:

葡萄糖杆菌氧化素的存在 1,3-二二氧化乙是一种脱水酶基因的基因料批发发酵的发酵方法代谢工程是代谢工程.

更多相关视频

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

Glycolysis: Preparatory Phase

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...

Products of the Citric Acid Cycle

Products of the Citric Acid Cycle

The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.

Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis

Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis

Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an...