持续的硫化物供应增强了Clostridium ragsdalei的酒精的自性生产
Luis Oliveira1, Simon Röhrenbach1, Verena Holzmüller1
1Department of Energy and Process Engineering, School of Engineering and Design, Chair of Biochemical Engineering, Technical University of Munich, Boltzmannstr. 15, 85748, Garching, Germany.
合成气的Clostridium ragsdalei发酵通过优化pH值和温度来提高乙醇生产. 持续的硫化养显著提高了乙醇产量和酒精与乙酸盐的比率.
科学领域:
- 微生物生物技术 微生物生物技术
- 生物化学工程是生物化学工程.
- 合成生物学 合成生物学
背景情况:
- 使用克洛斯特里迪亚的自营发酵将合成气 (CO,CO2,H2) 转化为有价值的有机酸和酒精.
- Clostridium ragsdalei 是用于合成气体发酵的关键微生物.
研究的目的:
- 为了研究Clostridium ragsdalei用于合成气发酵的批量工艺性能.
- 为了优化反应条件,包括pH值,温度和硫的可用性,以最大限度地提高乙醇的生产.
主要方法:
- 在连续加气的水箱生物反应器中批量发酵Clostridium ragsdalei.
- 系统变化的pH值 (6.0-5.5) 和温度 (37°C至32°C).
- 通过废气中的氨酸度和H2S监测硫的可用性;实施连续硫化物养.
主要成果:
- 在37°C下降pH值从6.0到5.5时,乙醇度从2.33增加到3.95g/L.
- 在pH值6.0的温度从37°C降低到32°C,在5天后产生了5.34g/L的乙醇.
- 不断食硫化使乙醇度增加到7.67g/L,而酒精与乙酸盐的比率增加到17.71g/g.
结论:
- 优化pH值和温度对于提高Clostridium ragsdalei合成气发酵中的乙醇产量至关重要.
- 硫的可用性是限制因素,持续补充硫化可以显著提高乙醇生产和产品形象.
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