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在Trichoderma reesei中通过Sr²⁺诱导细胞酶的产生,通过信号转导

Ni Li¹, Yi Zeng¹, Yumeng Chen¹

¹The State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Bioresources and bioprocessing

|April 22, 2024

概括

离子 (Sr2+) 通过激活信号通路来增强 Trichoderma reesei 中的细胞酶的产生. 然而,Sr2+也增加了活性氧物种 (ROS),这可能会阻碍酶的产生,这种负面影响被N-乙半氨酸 (NAC) 减轻.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
酶工程是什么? 酶工程是什么?

背景情况:

特里科德尔玛reesei RUT-C30是细胞酶生产的关键菌株,对于纤维素蛋白转化至关重要.
离子作为二次信使,调节T. reesei的生长和细胞酶合成.
了解调节细胞酶生产的因素对于优化工业应用至关重要.

研究的目的:

研究离子 (Sr2+) 对T. reesei RUT-C30.的细胞酶产生的影响.
阐明潜在的分子机制,包括信号传递和反应性氧物种 (ROS) 的参与.
为了确定细胞酶合成的潜在新型诱导剂.

主要方法:

补充T. reesei RUT-C30培养物的 Sr2+ .
测量细胞酶活性和基因表达.
细胞内度和信号通路 (Ca2+-CRZ1) 的分析.
使用通道阻塞剂 (LaCl₃) 和ROS清除剂 (NAC).

主要成果:

Sr2+显著增加细胞酶活性和调高细胞酶相关基因.
Sr2+增强了细胞质水平,激活了Ca2+-氨酸敏感指转录因子1 (CRZ1) 途径.
2+补充抑制了殖民地生长和增加了细胞内ROS,不利于细胞酶的产生.
ROS对细胞质生产的负面影响被NAC减轻.

结论:

Sr2+通过信号通路在T. reesei中刺激细胞酶的产生和基因表达.
由Sr2+诱导的细胞内ROS增加会对细胞酶的产生产生产生负面影响,但可以控制.
这项研究为细胞酶合成提供了机理性的见解,并将Sr2+确定为一种新型诱导剂.

关键词:

三甲虫reesei 在线阅读信号传递的.细胞质细胞质细胞质.这就是ROSOS ROS.信号传导传导是指信号的传导.在 Sr2++中.

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