来自白微生物群的新型GH10性热稳定型西兰酶的表征
Maria Laura Mon1, Rubén Marrero Díaz de Villegas1, Eleonora Campos1
1Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Hurlingham, Buenos Aires, Argentina.
Bioresources and bioprocessing
|April 22, 2024
概括
来自白肠微生物的新型GH10氧化酶 (Xyl10B) 在广泛的温度和pH范围中表现出高稳定性. 这种热性酶对工业应用有前途,如纸漂白和生产益生菌或生物乙醇.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 微生物生物技术 微生物生物技术
背景情况:
- 克西兰酶对于分解植物生物质的主要组成部分 - - 克西兰而言至关重要.
- 工业应用需要具有增强稳定性的新型酶.
- 长肠道微生物组是独特酶的丰富来源.
研究的目的:
- 描述一种来自虫肠道微生物组的热稳定型GH10氧化酶 (Xyl10B) 的新型特征.
- 评估其生物化学特性和分子结构特征.
- 评估其对工业应用的潜力.
主要方法:
- 枪支元基因组测序来识别西兰酶基因.
- 在大肠杆菌中基因克隆和异质表达.
- 净化和描述重组酶的活性和稳定性.
主要成果:
- 在pH 4-10和温度37-60°C之间,Xyl10B表现出广泛的活性,pH 9和50°C是最佳的.
- 在50°C下,酶在8小时内保持了80%以上的活性.
- 在盐,离子和工业化学品的存在下,Xyl10B表现出稳定性.
结论:
- Xyl10B是一种高度稳定的,热性GH10氧化酶,适用于纸和纸.
- 它的水解产物 - - 糖和糖 - - 具有作为益生菌和用于生物乙醇生产的潜力.
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