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在黑茶废弃物固态发酵中,Aspergillus glaucus优化化酶的产生

Moataza Mahmoud Saad¹, Abdelnaby Mahmoud Saad¹, Helmy Mohamed Hassan¹

¹Microbial Chemistry Department, National Research Centre (NRC), 33 Bohouth St, Dokki, 12622, Giza, Egypt.

Bioresources and bioprocessing

|April 22, 2024

概括

研究人员通过固态发酵 (SSF) 从黑茶废物中使用Aspergillus glaucus优化了酸酶的生产. 这项研究实现了高酸产量,证明了工业应用的高效化酶活性.

科学领域:

生物技术是生物技术.
酶技术酶技术是一种
微生物发酵微生物发酵

背景情况:

酸酶是重要的工业酶,在食品,制药,化品,皮革和环境生物技术中具有应用.
来自埃及土壤和海洋样本的真菌分离物被选,以检测其化酶生产能力.

研究的目的:

为了识别和表征一种高酶生成的真菌分离物.
用黑茶废物优化固态发酵 (SSF) 条件,用于使用黑茶废物生产化剂.
为了评估产生的化酶在将酸转化为酸的效率.

主要方法:

对15种真菌分离物进行选,以检测其化酶活性.
通过使用18S rRNA测序来识别最佳分离物 (NRC8) 作为阿斯伯吉勒斯格劳库斯.
优化SSF参数,包括水分含量,注射剂大小,酸度,pH,温度和化时间.
应用Box-Behnken设计和响应表面方法来进一步优化.
通过将酸转化为酸来评估酶活性.

主要成果:

阿斯伯吉卢斯格劳库斯 (Aspergillus glaucus) 隔离物NRC8表现出最高的酶活性.
黑茶废弃物被证明是通过SSF生产化酶的最有效基质.
确定了最佳的SSF条件:75%的水分,6×108个子/毫升的注射剂,0.2%的酸盐,pH值为5.0,在30°C下5天.
为了进一步优化,开发了一种显著的二次模型.
由黑茶废物产生的A. glaucus化酶产生了38.27毫克/毫升的酸.
在40°C的温度下观察到最佳的生物转化效率,酸度高达200g/L.

结论:

阿斯伯吉勒斯格劳库斯是酶酶的强有力的来源.
使用黑茶废弃物的优化SSF提供了一种高效和具有成本效益的化酶和酸生产方法.
描述的酶酶显示出工业应用的巨大潜力,特别是在生物转化过程中.

关键词:

非洲毒素测试阿斯伯吉勒斯 (Aspergillus glaucus) 黄糖酸是一种酸.剩余的茶叶剩余的茶叶化 (tannase) 是一种化剂.坦尼尼坦尼尼是一种

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