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来自Pseudarthrobacter sp.的冷活性三糖合成酶的表征用于三糖生物生产

Srisakul Trakarnpaiboon¹, Benjarat Bunterngsook¹, Hataikarn Lekakarn²

¹Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueang, Khlong Luang, Pathumthani, 12120, Thailand.

Bioresources and bioprocessing

|April 22, 2024

概括

一种新型的冷活性三糖合成酶 (PaTreS) 在单个步骤中有效地从马尔中产生三糖. 这种酶提供了一种有前途的低温生物催化方法,用于减少副产品的三糖生产.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
碳水化合物化学碳水化合物化学

背景情况:

糖是一种有价值的功能糖,在食品,化品和药品中具有多种应用.
传统的三糖生产涉及两步过程,效率较低,产生更多的副产品.
使用三合成酶 (TreS) 酶化,单步转换马尔糖为三糖,提供了一个简化和更清洁的替代方案.

研究的目的:

为了表达和描述来自Pseudarthrobacter sp.的冷活性三合成酶 (PaTreS). TBRC 2005 年的研究报告.
评估PaTreS作为生物催化剂用于低温三糖生产的潜力.

主要方法:

PaTreS.的异质表达和生物化学特征.
确定最佳温度,pH值和辅助因子要求.
使用PaTreS.高度麦芽糖糖的酶转化.

主要成果:

PaTreS在20°C时表现出最大活性,在较低的温度下保持显著活性 (4°C时59%).
该酶在广泛的pH范围 (7.0-9.0) 中表现出稳定性,并被双价金属离子 (Mg2+,Mn2+,Ca2+) 增强.
100-300g/L麦芽糖的转换在16小时内产生了71.7-225.5g/L三糖,其中葡萄糖副产品最小.

结论:

PaTreS 是一种高效的冷活性酶,用于从麦芽糖中单步生产三糖.
PaTreS的低温活性和副产品特征使其成为工业应用的有希望的生物催化剂.
这种酶方法在降低污染风险和简化下游加工以制造三糖方面具有优势.

关键词:

伪甲状腺细菌spsp.冷活酶是一种冷活酶.马尔托糖 (Maltose) 是一种葡萄糖.这种树脂含有三糖.三糖合成酶的使用方法

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