抗Acanthamoeba金属制药:铜的杀菌活性和协同作用 (II) 协调化合物
Jaqueline da Silveira1, Ana Paula Cardoso2, Christiane Fernandes2
1Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Laboratório de Investigação Aplicada a Protozoários Emergentes (LADIPE), Florianópolis, Santa Catarina, 88040-970, Brazil.
概括
铜 (II) 化合物对Acanthamoeba具有强烈的杀虫活性,这是严重眼部感染的原因. 化合物Cp12,与赫西丁相结合,为Acanthamoeba角膜炎提供了一个有前途的治疗选择.
科学领域:
- 眼科医生 眼科 眼科
- 传染性疾病 传染性疾病
- 药用化学 医学化学
背景情况:
- 阿坎萨摩巴菌种是微生物角膜炎的重要原因,导致角膜移植和视力丧失.
- 目前针对阿坎萨摩巴角膜炎的治疗方法具有低效率,高毒性,需要组合疗法.
研究的目的:
- 评估新型铜 (II) 协调化合物的对抗Acanthamoeba castellanii的氨杀菌疗效.
- 评估这些化合物的细胞毒性,以潜在的局部眼部应用.
- 为了研究铜 (II) 化合物与化的协同作用.
主要方法:
- 对铜 (II) 协调化合物的查,以防止Acanthamoeba castellanii (ATCC 50492) .
- 使用子角膜细胞系 (ATCC-CCL60) 进行细胞毒性评估.
- 确定最小抑制性氨基杀菌度 (MIAC) 和IC50值.
主要成果:
- 铜 (II) 化合物表现出显著的杀性活性,抑制了80%以上的矿的活力.
- 化合物Cp12和Cp13在200μM时表现出MIAC,IC50值低于10μM.
- Cp12显示了48%的活力降低对阿坎萨莫巴囊和与赫西丁的协同效应.
结论:
- 铜 (II) 协调化合物具有强烈的氨杀性和低细胞毒性,表明可能用于眼科配方.
- 化合物Cp12是治疗Acanthamoeba角膜炎和用于多用途隐形眼镜解决方案的有希望的候选物.
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