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Updated: Jun 28, 2025

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Published on: January 16, 2012

利用陶酸作为一种有效的抛光策略,以在双特异抗体净化过程中去除与产品和工艺相关的杂质

Nattha Ingavat¹, Xinhui Wang¹, Jia Min Liew¹

¹Downstream Processing Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Bioresources and bioprocessing

|April 22, 2024

概括

陶酸 (CHT) 有效地净化双特异抗体 (bsAbs),达到超过97%的纯度和减少杂质. 此外,CHT还可以缓解聚合,其性能优于传统的阴离子交换染色学 (CEX).

科学领域:

生物制药工艺开发生物制药过程开发
蛋白质净化蛋白质净化
染色体学染色体学是一种染色学.

背景情况:

双特异性抗体 (bsAbs) 是有前途的治疗方法,但由于结构复杂性和杂质概况,它们存在下游处理挑战.
陶酸 (CHT) 是一种混合模式的色谱介质,具有双重结合能力 (金属亲和和离子交换),可用于蛋白质净化.

研究的目的:

为了研究CHT作为双特异抗体 (bsAb) 净化抛光步骤的有效性.
为了将CHT性能与bsAb杂质去除和聚合减轻的传统阴离子交换染色学 (CEX) 进行比较.

主要方法:

研究了三种模型双特异性抗体 (bsAbs) 的CHT抛光后蛋白A化物.
与阴离子交换染色学 (CEX) 相比,基准的CHT性能.
优化了CHT的负载后洗 (PLW) 条件,以增强低分子量 (LMW) 杂质的去除.

主要成果:

对不对称和对称的IgG类bsAbs.CHT抛光实现了至少97%的产品纯度.
与CEX相比,CHT显示出更高的聚合物清除率,产生低高分子量 (HMW) 杂质 (0.5%) 并减轻染色学诱导的聚合.
在CHT上优化PLW可将LMW杂质降低高达48%.

结论:

CHT是一种高效的抛光介质,用于双特异抗体 (bsAb) 净化,具有高纯度和优异的杂质清除能力.
与CEX相比,CHT显著降低了染色学诱导的聚合,这是bsAb处理的关键优势.
建议CHT的独特C站点合作机制来推动其杂质清除和聚合减轻能力.

关键词:

双特异性抗体双特异性抗体染色学诱导的聚合物.混合模式色谱学混合模式色谱学产品和工艺相关的杂质去除产品和工艺相关的杂质去除

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