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住院儿童白细胞激活模式:比较SARS-CoV-2,细菌感染和炎症病理

Léa Domitien Payet^1,2, Anne Sophie Bedin², Émilie Desselas¹

¹Department of General Pediatrics, Infectiology and Clinical Immunology, Arnaud de Villeneuve UHC, 371 Avenue du Doyen Gaston GIRAUD, 34295 Montpellier Cedex 5, France.

Journal of leukocyte biology

|April 22, 2024

概括

白细胞分析揭示了SARS-CoV-2感染,细菌感染或炎症性疾病的儿童的独特免疫细胞激活模式. 这有助于在儿科患者中区分这些疾病.

科学领域:

儿科免疫学儿科免疫学
传染性疾病传染性疾病
流动细胞计量 (Flow Cytometry) 是一种流动细胞计.

背景情况:

在成年人中,严重的SARS-CoV-2感染包括由单细胞和中性粒细胞介导的高炎症反应.
了解急性发烧性疾病儿童的免疫细胞激活对于准确诊断至关重要.

研究的目的:

评估和区分被诊断患有SARS-CoV-2感染,细菌感染或炎症疾病的儿童的白细胞激活模式.
确定特定的免疫细胞标记物,以区分这些儿科疾病.

主要方法:

流细胞计用于分析55名患有急性发烧的儿童的白细胞激活标志物.
分析的标记物包括单细胞上的CD169和HLA-DR,中性粒细胞上的CD64和CD16,以及TCD8淋巴细胞上的CD38.
患者被分为SARS-CoV-2感染,细菌感染和炎症性疾病组.

主要成果:

在所有感染SARS-CoV-2的儿童中观察到CD169在单细胞和CD38在T淋巴细胞上的过度表达.
对中性粒细胞的CD64过度表达是细菌感染和炎症性疾病的特征.
在细菌感染和炎症病理学组中注意到单细胞上HLA-DR表达的减少.

结论:

使用特定细胞表面标记物的白细胞分析可以有效地区分SARS-CoV-2感染,细菌感染和儿童的炎症性疾病.
独特的免疫激活档案为儿科发烧性疾病提供了宝贵的诊断见解.

关键词:

这就是SARS-CoV-2病毒.生物标志物生物标志物孩子孩子孩子孩子孩子孩子在白细胞激活过程中,

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