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在早期的Teleost进化过程中,保存的非编码元素的大量损失和收益
Elisavet Iliopoulou1,2, Vasileios Papadogiannis1,3, Costas S Tsigenopoulos1
1Hellenic Centre for Marine Research (HCMR), Institute of Marine Biology, Biotechnology & Aquaculture (IMBBC), Heraklion, Greece.
Genome biology and evolution
|April 22, 2024
概括
远距离生物中保存的非编码元素在进化早期显示出显著的损失,但在远距离生物干群中也获得了巨大的收益. 这些新获得的元素对发育至关重要,并可以帮助远古遗传学.
科学领域:
- 进化基因组学是进化的基因组学.
- 进行比较的基因组学.
- 发育生物学是发展生物学.
背景情况:
- 保存的非编码元素 (CNEs) 是脊椎动物中重要的调节区域,通常位于发育基因附近.
- 之前的研究表明,CNE损失和快速分歧的teleosts,但有限的基因组数据.
研究的目的:
- 通过使用一套扩展的高质量的teleost基因组来研究远距离保存的非编码元素的进化.
- 为了识别和描述整个远端进化历史的CNE收益和损失动态.
- 评估CNE对远古遗传学的有用性.
主要方法:
- 针对性基因组对齐和对比跨越远远的基因组.
- 基于合成的关联,将CNE与其假定调节的目标基因联系起来.
- 在远距离的CNE中分析动机和转录因子结合站点词汇.
主要成果:
- 证实了脊椎动物CNE在远进化早期的大量丧失.
- 在3亿多年前的远干群中发现了CNE的大量增长.
- 确定了最近获得的近距离的CNE,靠近发育和转录调节基因,与脊椎动物CNE共享类似的调节词汇.
- 证明了泛-teleost CNEs对于准确的遗传学定位的潜力.
结论:
- 早期的teleost CNE演变既涉及显著的损失和实质性的收益,可能是通过分歧和de novo出现.
- 新获得的CNE在teleost特定基因调控中发挥作用,特别是在发育过程中.
- 全teleost CNEs代表了解决teleost进化关系的有价值标记.
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