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生物质酸预处理对暗发酵过程的代谢途径和细菌成分的影响

K Dauptain¹, E Trably¹, G Santa-Catalina¹

¹INRAE, Université de Montpellier, LBE, 102 avenue des Étangs, 11100 Narbonne, France.

Waste management (New York, N.Y.)

|April 22, 2024

概括

复杂有机物质的热酸预处理改善了糖的溶解,但没有通过暗发酵 (DF) 增强的产生. 然而,它通过影响本地细菌改变了细菌群落和代谢途径.

科学领域:

生物技术是生物技术.
环境科学环境科学
生物化学生化学

背景情况:

复杂的有机物质是通过暗发酵 (DF) 来生产的可行基质.
预处理技术通常是必要的,以优化这些基板的产量.
了解预处理对基质特征和微生物群落的影响对于高效的生物过程至关重要.

研究的目的:

评估热酸预处理对和城市固体废物有机部分 (OFMSW) 中糖溶解的作用.
评估热酸预处理对暗发酵 (DF) 性能的影响,包括 (H₂) 和代谢物生产.
研究DF中热酸预处理引起的细菌群体和代谢概况的变化.

主要方法:

生物化学潜力测试使用原料和热酸预处理的和OFMSW进行.
在没有添加外部注射剂的情况下进行了测试,以关注基质预处理对土著微生物群体的影响.
分析了细菌群体组成和代谢概况,以了解预处理的影响.

主要成果:

热酸预处理显著改善了和OFMSW的糖溶解.
尽管增加了糖溶解,但DF性能 (H₂产量和总代谢物产量) 在预处理和原始生物质之间是可比的.
预处理引发了细菌群落的显著转变,从肠杆菌群转向闭膜菌群和细菌群,并改变了从酸盐到酸盐或乙醇的代谢输出.

结论:

热酸预处理在增加糖溶解方面是有效的,但在使用这些基质的DF中不会直接增强的生产.
观察到的细菌和代谢概况的变化主要归因于预处理对生物质内的本地细菌的影响,而不是对发酵过程本身的影响.
可能需要进一步的研究来优化预处理策略或选择特定的微生物联盟,以利用改善的糖可用性增加产量.

关键词:

酸性预处理酸性预处理细菌群体是细菌群体.生物气是生物气中的一种.黑暗发酵黑暗发酵土著细菌是土著的细菌.

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