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CLI:一种用于从复杂植物样本中分离乳酸细菌的新协议

Mariannys Chavez¹, Sharon Viscardi², María José Ruiz³

¹Doctoral Program in Natural Bioresource Sciences, Universidad de La Frontera, Temuco 4811230, Chile; Biocontrol Research Laboratory, Universidad de La Frontera, Temuco 4811230, Chile.

Journal of microbiological methods

|April 22, 2024

概括

一种新的方法,复杂样本LAB隔离 (CLI),有效地从植物组织中隔离乳酸细菌 (LAB). 该协议促进了对LAB用于农业应用的研究.

科学领域:

微生物学微生物学
植物科学植物科学
农业科学农业科学

背景情况:

乳酸细菌 (LAB) 是公认的益生菌,通常在人类肠道和发酵食品中发现.
最近的发现表明,LAB也存在于植物微生物群中,显示出植物的益生菌潜力.
越来越多的科学兴趣集中在为农业应用隔离LAB上,但缺乏用于植物样本的高效协议.

研究的目的:

从复杂的植物样本中分离乳酸细菌 (LAB) 的新型高效协议的开发和验证.
建立一种可靠的方法来获得纯化的LAB,用于进一步的农业研究.

主要方法:

开发的协议,复杂样本LAB隔离 (CLI),涉及五个关键步骤:预化,均质化,连续稀释,在MRS上选择性培养特定添加剂 (酸,CaCO3,受控氧气,pH5.8),和殖民地选择.
在微气友条件下 (O2 <15%) 的化条件包括37°C48小时.
选择标准侧重于典型的LAB形态和存在CaCO3溶解环的存在.

主要成果:

该CLI协议显示了足够的灵敏度,以从植物组织中分离代表性的LAB属,包括Leuconostoc,Lactococcus和Enterococcus.
该方法成功地产生了具有特征形态和碳酸溶解 halos 的净化 LAB 殖民地.

结论:

CLI协议提供了一种高效和灵敏的方法,可以从复杂的植物基质中分离乳酸细菌.
这个新协议是对未来研究本地LAB及其在农业中的潜在应用的有价值的工具.

关键词:

复杂的样品复杂的样品乳酸细菌乳酸细菌乳酸细菌新方法新方法的方法.植物益生菌是一种植物益生菌.

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