内镜采样和培养方法
1Medical Device Testing, Eurofins Biotech Germande, Aix-en-Provence, France.
The Journal of hospital infection
|April 22, 2024
概括
由于污染率的广泛变化,标准化灵活的内镜取样和培养至关重要. 这项研究发现,各种方法在微生物恢复效率上存在显著差异,这凸显了需要采用统一方法的需要.
科学领域:
- 微生物学 微生物学
- 感染控制 感染控制
- 医疗设备再加工 医疗设备再加工
背景情况:
- 在文献中,患者准备的灵活内镜污染率差异很大 (0.4%-49%).
- 不一致的采样,培养方法和定义阻碍了结果的解释.
- 缺乏标准化使得对内镜污染水平的比较变得复杂.
研究的目的:
- 为了比较六种十二指镜采样和培养方法的疗效.
- 确定从内镜中获得最佳微生物恢复的关键参数.
- 为了解决灵活的内镜污染评估中的变异性.
主要方法:
- 使用重复恢复方法 (ISO 11737-1:2018) 评估了十二指镜样本提取效率.
- 评估了六种不同的采样和培养技术.
- 专注于微生物回收和提取效率.
主要成果:
- 平均生物负担提取效率在1% (澳大利亚方法) 到39% (法国方法) 之间.
- 效率较低与中和剂,摩擦或张力活性剂的缺失有关.
- 疗效取决于微生物的类型和从取样到培养的时间.
结论:
- 目前的灵活内镜采样和培养方法存在显著的变化.
- 需要一种协调和标准化的方法来进行灵活的内镜采样和培养.
- 标准化将提高污染数据的可靠性和可比性.
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