染色体规模的基因组组装和Cotoneaster Glaucophyllus的注释
Kaikai Meng1,2, Wenbo Liao1, Shaolong Wei2
1State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Scientific data
|April 22, 2024
概括
使用先进的测序技术,为Cotoneaster glaucophyllus生成了染色体规模的基因组组. 这为了解其装饰性和生态重要性以及未来的基因组研究提供了宝贵的资源.
科学领域:
- 植物基因组学 植物基因组学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 科托尼阿斯特 (Cotoneaster glaucophyllus) 是一种半常青植物,因其装饰性和生态贡献而受到重视.
- 它的芳香的白色花朵在夏末开花.
- 基因组资源对于理解植物进化和功能至关重要.
研究的目的:
- 为了生成高质量的染色体规模的基因组组装,用于Cotoneaster glaucophyllus.
- 为物种的分子和进化研究提供基础资源.
- 为了促进功能基因组学和罗萨ceae家族内的比较基因组学.
主要方法:
- 整合了PacBio和Illumina测序数据.
- 使用Hi-C技术进行染色体支架.
- 用于基因预测和注释的生物信息分析.
主要成果:
- 一个563.3 Mb的基因组组装得到了与架N50的~31 Mb.
- 95.59%的序列被固定在17个伪染色体上.
- 预测了35,856个编码蛋白质的基因和各种非编码RNA.
结论:
- 染色体水平的基因组组合代表了Cotoneaster Glaucophyllus研究的重大进展.
- 这个资源将加速物种化,功能基因组学和比较基因组学研究.
- 它增强了我们对罗莎系植物家族遗传多样性的理解.
更多相关视频
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
10.5K
13:03Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
10.6K
相关概念视频
Genome Annotation and Assembly
18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Lampbrush Chromosomes
7.9K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
7.9K
Karyotyping
60.1K
Overview
60.1K
Assembly of Cytoskeletal Filaments
19.5K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
19.5K
Condensins
3.5K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.5K
Polytene Chromosomes
10.0K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.0K
