高时空带宽产品成像在低连贯度定量相位显微镜
Azeem Ahmad1, Paweł Gocłowski2, Vishesh Dubey2
1Department of Physics and Technology, UiT The Arctic University of Norway, 9037, Tromsø, Norway. ahmadazeem870@gmail.com.
Scientific reports
|April 22, 2024
概括
本研究引入了低连贯量相显微镜 (LC-QPM) 的混合方法,增强了空间和时间分辨率. 这种新的方法使用不连贯的光和希尔伯特螺旋转换 (HST) 算法进行高速度,高灵敏度的生物样本成像.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 光学显微镜的使用方法
- 定量阶段显微镜技术
背景情况:
- 低相干量相显微镜 (LC-QPM) 面临视野 (FoV) 和时间分辨率之间的权衡,因为光源的时间相干性 (TC) 是有限的.
- 现有的LC-QPM系统难以同时实现高空间分辨率,高相位灵敏度和高时间分辨率.
研究的目的:
- 开发一种混合实验和数值方法,以克服当前LC-QPM系统的局限性.
- 通过增强空间和时间分辨率,在LC-QPM中实现高时空带宽产品.
主要方法:
- 在QPM中使用不连贯的光源进行样本照明,以提高空间分辨率.
- 利用基于希尔伯特螺旋转换 (HST) 的单次相恢复算法来增强时间分辨率,而不会影响空间分辨率.
- 集成的HST与LC-QPM用于可扩展的FoV和单拍成像中的分辨率.
主要成果:
- 在高时间分辨率下在LC-QPM中展示了高空间分辨率和高相位灵敏度.
- 与单拍里叶变换 (FT) 方法相比,实现了更高的空间分辨率,克服了边缘密度限制.
- 成功成像活体和固定生物标本,包括MEF,U2OS和人类红细胞 (RBC).
结论:
- 混合LC-QPM系统与HST重建使高速,单拍成像具有高相传感度和空间分辨率.
- 这种方法允许对亚细胞动态进行扩展观察,并对细胞过程进行高速成像.
- 这种方法为先进的生物医学成像应用提供了一个有希望的新方向.
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