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从叶子中提取可溶性蛋白质

Elizabete Carmo-Silva1, Rhiannon Page2, Catherine J Marsden2

  • 1Lancaster Environment Centre, Lancaster University, Lancaster, UK. e.carmosilva@lancaster.ac.uk.

Methods in molecular biology (Clifton, N.J.)
|April 22, 2024
PubMed
概括

本章详细介绍了从叶片样本中提取植物蛋白的方法. 它强调保护蛋白质完整性,用于分析植物生物化学中的丰富性和酶活性.

关键词:
卡尔文-本森-巴什姆的循环周期хлоропласт蛋白质是一种质蛋白质.酶活性的酶活性.光合作用的新陈代谢.蛋白质的生物化学 蛋白质的生物化学蛋白质完整性 蛋白质完整性蛋白质量化量化的蛋白质.鲁比斯科 (Rubisco) 是一种古老的葡萄酒.鲁比斯科可以激活Rubisco激活酶.

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Extraction: Advanced Methods00:56

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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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