Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Viscosity01:17

Viscosity

5.9K
When water is poured into a glass, it falls freely and quickly, whereas if honey or maple syrup is poured over a pancake, it flows slowly and sticks to the surface of the container. This difference in the flow of different kinds of liquids arises due to the fluid friction between the liquid layers and the liquid and the surrounding material. This property of fluids is called fluid viscosity. In this example, water has a lower viscosity than honey and maple syrup.
The SI unit of viscosity is...
5.9K
Surface Tension, Capillary Action, and Viscosity02:57

Surface Tension, Capillary Action, and Viscosity

27.7K
Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
27.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hierarchical classification of immune cell transcriptomes at population-scale.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Age-related epithelial defects limit thymic function and regeneration.

Nature immunology·2026
Same author

Terminal Conjugation Enables Nanopore Sequencing of Peptides.

Journal of the American Chemical Society·2026
Same author

High-throughput single-cell omics using semipermeable capsules.

Science (New York, N.Y.)·2025
Same author

Author Correction: Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes.

Nature microbiology·2025
Same author

Transcriptomic Plasticity Is a Hallmark of Metastatic Pancreatic Cancer.

Cancer research·2025
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
查看所有相关文章

相关实验视频

Updated: Jun 28, 2025

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

18.7K

增加流体粘度可确保一致的单细胞封装.

Emile Pranauskaite1, Valdemaras Milkus1, Justas Ritmejeris1

  • 1Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT 10257, Lithuania.

Analytical chemistry
|April 22, 2024
PubMed
概括
此摘要是机器生成的。

增加微流体中的液体粘度可以提高单细胞隔离效率. 这种方法可确保对高通量分析进行一致的细胞捕获,克服由细胞变性引起的偏差.

更多相关视频

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.7K
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
10:20

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

19.6K

相关实验视频

Last Updated: Jun 28, 2025

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

18.7K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.7K
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
10:20

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

19.6K

科学领域:

  • 生物技术是生物技术.
  • 微流体学 微流体学
  • 细胞生物学 细胞生物学

背景情况:

  • 高通量单细胞分析需要隔离细胞进行表征.
  • 微流体设备通常使用微滴或微来隔离细胞.
  • 细胞特性 (大小,形状,密度) 的变化导致不一致的捕获效率和分析工件.

研究的目的:

  • 在微流体学中研究规避单细胞分离偏差的方法.
  • 为了确定增加液体粘度是否可以改善细胞捕获的一致性.
  • 为了比较粘度调整与细胞密度调整的有效性.

主要方法:

  • 使用微流体芯片在油中的水滴中进行单细胞封装.
  • 在粘度增加 (40-50 cP) 的液体中分散细胞.
  • 随着时间的推移,监测细胞流量和封装效率.

主要成果:

  • 增加的液体粘度显著减少了细胞沉积.
  • 在微流体芯片内实现了一致的细胞流动.
  • 几乎所有细胞都能以均的效率被分离出来,不论细胞类型如何.
  • 这种方法减轻了与细胞可变性相关的偏差.

结论:

  • 增加液体粘度是克服微流体学中单细胞隔离偏差的可靠策略.
  • 这种方法提高了高通量单细胞分析的准确性,特别是在异质种群中.
  • 粘度调整提供了一个比改变细胞密度更强大的解决方案,以实现一致的细胞隔离.