多重蛋白质组学识别了与炎症相关的血生物标志物,用于衰老和心脏代谢障碍
Siting Wu1, Yulin Li1, Xue Zhao1
1Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, 300070, China.
Clinical proteomics
|April 22, 2024
概括
像EGF和MMP1这样的炎症蛋白质是衰老和心脏代谢障碍 (CMD) 的关键. 十个重叠的蛋白质显示CMD在衰老中的诊断价值,有助于了解疾病的进展.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 分子生物学分子生物学
- 老年学是指老年学的学科.
背景情况:
- 心脏代谢障碍 (CMD) 在老年人群中很普遍,是心血管疾病 (CVD) 的重要危险因素.
- 炎症在心血管疾病和衰老的发展中起着作用,但具体的炎症分子表型尚不清楚.
研究的目的:
- 确定与正常衰老和CMD相关的炎症分子表型.
- 在老龄化人口中探索CMD的潜在蛋白质生物标志物.
主要方法:
- 利用近距离延伸试验 (PEA) 来分析来自年轻人,老年人和患有CMD的老年人血中的368种炎症蛋白.
- 构建了蛋白质-蛋白质相互作用 (PPI) 网络,以在差异表达蛋白 (DEP) 中识别枢纽蛋白.
- 评估了已识别的蛋白质和临床特征之间的相关性,并使用ROC曲线分析评估了诊断价值.
主要成果:
- 在正常衰老中确定了161个DEP (枢纽蛋白:EGF) 和28个在患有CMD的老年人中发现的DEP (枢纽蛋白:MMP1).
- 发现10种重叠的蛋白质 (例如,SHMT1,EGLN1,NCF2) 与脂质和葡萄糖水平有显著的相关性.
- 这10种蛋白质在老龄化中显示出CMD的良好诊断价值 (AUC>0.7),EGLN1,NCF2,REG4和SLC39A2在两个老龄化群体中都增加.
结论:
- 该研究确定了正常衰老和CMD的潜在分子标志物.
- 需要进一步研究更大的样本大小来验证这些发现,并预测它们对心血管疾病的意义.
更多相关视频
08:51Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
Published on: September 20, 2024
1.2K
09:43Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging
Published on: January 10, 2025
1.2K
相关概念视频
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
81
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
81
01:33Proteomics
7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
144
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
144