一种番茄NAC转录因子SlNAP1直接调节依赖吉伯雷林的水果成熟
Changxia Li1,2, Xuemei Hou1, Zongxi Zhao1
1College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou, 730070, China.
Cellular & molecular biology letters
|April 22, 2024
概括
番茄果实成熟过程受到NAC转录因子SlNAP1.1的积极调节. SlNAP1激活了吉伯雷林降解基因,延迟了成熟. 这项研究揭示了番茄果子发育中的关键分子机制.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 番茄 (Solanum lycopersicum) 的果实成熟是一个复杂的过程,由特定的基因表达控制,由转录因子 (TF) 调节.
- NAC TF家族在各种植物生理过程中发挥着至关重要的作用,包括果实成熟,但它们在番茄中的特定功能和基因在很大程度上仍未被描述.
研究的目的:
- 调查NAC TF SlNAP1在调节番茄果实成熟 (FR) 的作用.
- 确定SlNAP1的直接标基因,并阐明其通过吉伯雷林 (GA) 途径控制FR的分子机制.
主要方法:
- 在番茄中使用CRISPR/Cas9基因编辑生成slnap1突变体.
- 酵母一杂交 (Y1H),双光酶记者 (DLR),酵母两杂交 (Y2H),双分子光补充 (BIFC) 和光酶 (LUC) 试验被用于确定基因促进体相互作用和蛋白质蛋白质相互作用.
主要成果:
- 与野生类型 (WT) 相比,slnap1突变体表现出延迟水果成熟的表型,包括减少乙烯生产和更慢的颜色变化.
- 证实SlNAP1直接结合并激活SlGA2ox1和SlGA2ox5的促进子,这些基因是吉伯雷林降解中的关键基因.
- 发现SlNAP1与SlGID1相互作用,SlNAP1与SlGID1相互作用,SlNAP1与SlgID1相互作用,SlgID1与SlgID1相互作用,SlgID1与SlgID1相互作用,SlgID1与SlgID1相互作用,SlgID1与SlgID1相互作用.
结论:
- SlNAP1通过直接激活吉伯雷林降解基因,积极调节番茄果实的成熟.
- SlNAP1 和 SlGID1 之间的相互作用可能会导致 SlNAP1 介导的水果成熟.
- 这项研究为NACTFs通过GA通路调节番茄FR的分子机制提供了重要的见解.
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