下呼吸道微生物群的组成在流感,COVID-19和与细菌有关的急性呼吸困扰综合征之间有所不同
Sébastien Imbert1,2, Mathilde Revers1,2, Raphaël Enaud2,3
1HU Bordeaux, Mycology-Parasitology Department, CIC 1401, 33000, Bordeaux, France.
Critical care (London, England)
|April 22, 2024
概括
下呼吸道微生物群的组成在急性呼吸困难综合征 (ARDS) 的各种原因中显著不同,包括流感,COVID-19和细菌性肺炎. 了解这些微生物差异对于ARDS研究至关重要.
科学领域:
- 微生物学 微生物学
- 肺部病理学 肺部病理学
- 传染性疾病 传染性疾病
背景情况:
- 急性呼吸困难综合征 (ARDS) 每年造成40万人的死亡,由于其异质性质,治疗进展有限.
- 下呼吸道微生物群在ARDS病理生理学中的作用越来越被认可,但ARDS病因学的变异仍然不清楚.
研究的目的:
- 为了研究和比较由流感,SARS-CoV-2或细菌感染引起的ARDS患者的下呼吸道微生物组成.
主要方法:
- 从53名ARDS患者 (24名COVID-19,18名流感,11名细菌CAP) 的内气吸液分析,需要侵入性通风.
- 深度测序V3-V4和ITS2区域以表征细菌和真菌群落.
- 使用DADA2管道对微生物多样性 (α和β多样性) 的统计分析.
主要成果:
- 在三种ARDS病因 (分别p=0.05和p=0.01) 之间观察到下呼吸道细菌和真菌微生物群组成 (β多样性) 的显著差异.
- 与COVID-19 ARDS相比,与细菌CAP相关的ARDS中的细菌α多样性较低 (p=0.04).与COVID-19 ARDS相比,与细菌CAP相关的ARDS中的细菌α多样性较低 (p=0.04).
- 在流感相关的ARDS中,真菌α多样性高于COVID-19的ARDS (p<0.01).
结论:
- 包括细菌和真菌在内的下呼吸道微生物群的组成因ARDS (流感,COVID-19,细菌性肺炎) 的潜在原因而异.
- 针对病因学的具体调查对于未来研究肺微生物群在ARDS病变发生中的作用至关重要.
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