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Updated: Jun 28, 2025

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circ_JMJD1C通过调节miR-182-5p/JMJD1C/SOX4轴加速乳腺癌的进展
Renyuan Xu1, Haomiao Lan2, Li Zhang3
1Department of Thyroid and Breast Surgery, Zigong First People's Hospital, Zigong 643000, Sichuan, China.. xry36512023@163.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|April 23, 2024
概括
循环RNAs (circRNAs) 在乳腺癌 (BC) 中发挥作用. 这项研究发现,circ_JMJD1C通过调节miR-182-5p/JMJD1C/SOX4轴来促进BC的攻击性,提供了一个潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为与各种癌症有关.
- 在乳腺癌 (BC) 进展中circ_0006743 (circ_JMJD1C) 的特定作用需要进一步阐明.
研究的目的:
- 研究 circ_JMJD1C 在乳腺癌中的功能和机制.
- 在BC中探索涉及circ_JMJD1C,miR-182-5p,JMJD1C和SOX4的调节轴.
主要方法:
- 生物信息分析以确定下游目标和交互网络.
- 基因表达分析 (Western blot,qRT-PCR),体外/体内功能测试,FISH和共聚焦显微镜.
- 路西法酶记者,RNA免疫沉降 (RIP) 和皮尔森的相关性分析以确定分子相互作用.
主要成果:
- 在乳腺癌组织中,Circ_JMJD1C和JMJD1C的表达率升高并与之有正相关.
- 抑制circ_JMJD1C抑制了BC细胞的增殖,入侵,迁移和上皮-介质细胞过渡 (EMT) 在体外和体内.
- Circ_JMJD1C通过miR-182-5p/JMJD1C/SOX4轴促进BC的攻击性,而circ_JMJD1C通过表观遗传上调SOX4.
结论:
- Circ_JMJD1C在乳腺癌进展中起到瘤基因的作用.
- 由circ_JMJD1C调节的miR-182-5p/JMJD1C/SOX4通路代表了乳腺癌的新治疗标.
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