通过性预处理和使用从分解草中分离出来的Bacillus菌株进行酶性水解,从草水解盐酸中获得的多酸酸的生产
Doan Van Thuoc1, Nguyen Thi Chung1, Rajni Hatti-Kaul2
1Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam.
Bioresources and bioprocessing
|April 23, 2024
概括
研究人员从大米中分离了能够产生多氧酸盐 (PHA) 的Bacillus物种. 用氨对草进行优化预处理,产生了大量的糖分释放,导致细菌产生大量的PHA.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 微生物学 微生物学
- 聚合物科学 聚合物科学
背景情况:
- 米是一种丰富的,低成本的纤维素生物质,具有生物经济的潜力.
- 可生物降解的聚合物,如聚化酸 (PHA),是石油塑料的可持续替代品.
- 有效利用农业废物用于生物聚合物生产对于环境可持续性至关重要.
研究的目的:
- 从米中分离出能够产生多氧酸盐 (PHA) 的细菌.
- 优化米的预处理,作为PHA发酵的碳来源.
- 用预处理的米来评估被分离的细菌产生的PHA.
主要方法:
- 使用16S rRNA基因测序从草中分离和识别产生PHA的细菌.
- 使用氧化,氧化和水性氨酸对草进行性预处理.
- 预处理的米的酶性水解以释放降解糖.
- 在预处理的米上发酵孤立的Bacillus物种,用于PHA生产.
主要成果:
- 七种产生PHA的细菌分离物被确定为Bacillus物种,包括Bacillus cereus,Bacillus paranthracis和Bacillus thuringiensis.
- 在80°C进行15小时的氨预处理,实现了最高的木质素去除 (63%) 和总减少糖释放 (92%).
- 细菌花菌株VK92和VK98获得了最高的PHA含量 (59.3%和46.4%) 和度 (2.96 g/L和2.51 g/L).
结论:
- 米可以作为一种双重用途的原料,既可以分离产生PHA的细菌,也可以作为PHA生物合成的碳来源.
- 优化的氨预处理显著增强了大米的糖化,提高了其适用于微生物发酵的适用性.
- 鉴定到的Bacillus cereus分离体显示出从农业废物中高效地生产聚酸的巨大潜力.
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