通过工程Yarrowia lipolytica从脂质中生产α-Farnesene
Yinghang Liu1, Zhaoxuan Wang1, Zhiyong Cui1
1State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Qingdao, 266237, People's Republic of China.
Bioresources and bioprocessing
|April 23, 2024
概括
这项研究提高了Yarrowia lipolytica中使用脂质原料的α-farnesene生产. 代谢工程和优化的发酵实现了高产量,证明了酵母的酵母.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 废脂原料为生产高价值化学品提供了可持续的资源.
- 雅罗维亚 (Yarrowia lipolytica) 是一种油性酵母,能够利用各种疏水基质.
- 阿尔法法尼是一种有价值的基烯,具有多种工业应用.
研究的目的:
- 从脂质原料中对Yarrowia lipolytica进行增强的α-farnesene合成.
- 识别和克服阿尔法法尼生物合成的关键限制因素.
- 为了评估酵母在各种废物和食用油上的性能.
主要方法:
- 雅罗维亚脂质菌的代谢工程,包括基因拷贝数放大 (ERG12,FSERG20) 和异质基因表达 (VHb).
- 用油酸作为基质优化发酵条件.
- 用于高密度细胞生长和产品积累的食批量培养.
- 使用葡萄糖与基于脂质的基质对alpha-farnesene生产的比较分析.
主要成果:
- 增加ERG12和FSERG20的副本数量增加了35.8%的α-法纳产量.
- 异质VHb的表达进一步提高了12.7%的产量.
- 达到最终的α-法纳标位为10.2g/L,产量为0.1g/g油酸.
- 与葡萄糖相比,脂质基质导致更高的产量和标位,并减少了副产品的形成.
结论:
- Yarrowia lipolytica是一个强大的平台,可以从脂质原料中生产alpha-farnesene.
- 代谢工程策略有效地改善了甲生物合成.
- 使用废油为化学生产提供了一个经济可行的和环保的方法.
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