通过A. pullulansFRR 5284的低成本甘糖蜜,高效地生产转果糖化酶
Most Sheauly Khatun1,2, Morteza Hassanpour1,2, Mark D Harrison1,3
1Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane, QLD, 4000, Australia.
Bioresources and bioprocessing
|April 23, 2024
概括
这项研究优化了使用Aureobasidium pullulans FRR 5284从甘糖蜜中转果糖化酶的生产. 优化条件显著增加了酶活性和生产力,使得可以控制果酸糖 (FOS) 配置,从而增强了益生菌的应用.
科学领域:
- 生物技术和工业微生物学
- 酶技术 酶技术是一种
- 益生菌生产 益生菌生产
背景情况:
- 果脂糖 (FOS) 是由转果糖酶产生的重要益生菌.
- 甘糖蜜为酶生产提供了一个可持续的基质.
- 优化酶生产对于高效的FOS合成至关重要.
研究的目的:
- 为了优化Aureobasidium pullulansFRR 5284使用甘糖蜜产生的转果酸酶的生产.
- 研究源和度对酶生产的影响.
- 评估扩展潜力和FOS生产能力.
主要方法:
- 使用了甘糖蜜作为A. pullulans FRR 5284种植的基质.
- 优化源 (NaNO3) 和度用于酶生产.
- 从摇瓶扩展到1L生物反应器.
- 评估了使用糖作为基质的酶活性和FOS产量.
主要成果:
- 与酵母提取物相比,NaNO3被确定为优越的源.
- 在糖蜜介质中,4.4 g/L的最佳NaNO3度产生了123.8 U/mL的酶活性.
- 扩展到生物反应器增加了39%的酶活性和108%的生产率.
- 以糖糖为基质,达到61%的最大FOS产量.
- 通过调整细胞内和细胞外酶比率来证明对FOS配置的控制.
结论:
- 优化的发酵条件显著提高了转果糖化酶的产生.
- 扩大规模是可行的,从而提高了酶活性和生产力.
- 该研究展示了一种方法,用于为特定的益生菌应用量身定制FOS配置文件.
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