一个比较研究的绿色替代品从甘包生产纳米纤维素
Bhargavi Pula1, Shradha Ramesh1, Sirisha Pamidipati1
1Department of Chemical Engineering, Birla Institute of Technology and Science Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, India.
Bioresources and bioprocessing
|April 23, 2024
概括
使用乳糖酶从甘中提取纳米纤维素的酶提取被葡萄糖氧化酶和ABTS增强. 这些调节器改善了素降解,产生了优化的纳米纤维素,使用葡萄糖氧化酶进行了更好的除.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 绿色化学 绿色化学
背景情况:
- 纳米纤维素的酶提取为传统方法提供了一个可持续的替代方案.
- 在纳米纤维素生产中,可使用辅助酶或介质来提高乳酸酶的效率.
研究的目的:
- 调查使用葡萄糖氧化酶和ABTS作为调节剂,以增强从甘包中拉卡斯介导的纳米纤维素提取.
- 为了优化葡萄糖氧化酶和ABTS的度,以改善纳米纤维素的特性.
主要方法:
- 使用乳糖酶提取纳米纤维素,单独添加葡萄糖氧化酶或ABTS.
- 对纤维素和素含量的分析,FT-IR,TGA,DSC,XRD,SEM和PSA.
- 基于红素降解,纤维素含量和结晶性的调节剂度的优化.
主要成果:
- 葡萄糖氧化酶和ABTS都改善了在木质素降解中的乳酶性能.
- 确定最佳度为ABTS的1.4mM和葡萄糖氧化酶的0.15mg/ml.
- 使用葡萄糖氧化酶提取的纳米纤维素显示出较小的尺寸 (339 nm) 和更好的除能力,与使用ABTS (636 nm) 获得的相比.
结论:
- 葡萄糖氧化酶和ABTS有效地增强基于乳糖酶的纳米纤维素提取.
- 葡萄糖氧化酶在从甘包中产生具有理想特征的纳米纤维素方面表现出卓越的性能.
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