优化培养基和循环养批次发酵策略,以提高Bacillus thuringiensis通过使用富含葡萄糖的水解酸盐来提高聚酸酸的生产
Sarisha Singh1, Bruce Sithole2,3, Prabashni Lekha2
1Discipline of Microbiology, University of KwaZulu-Natal (Westville Campus), Durban, South Africa. sarish_s@yahoo.com.
Bioresources and bioprocessing
|April 23, 2024
概括
这项研究引入了循环食批次发酵 (CFBF) 以促进使用Bacillus thuringiensis的多氧酸盐 (PHA) 生产. 这种方法显著提高了从基纤维素废物水解盐中获得的生物塑料产量和生产率.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 环境科学 环境科学
背景情况:
- 石化塑料废弃物对环境构成重大威胁.
- 聚氨基酸盐 (PHAs) 是可生物降解的生物塑料,有可能取代传统塑料.
- 高昂的生产成本和低产量阻碍了PHA生物塑料的广泛采用.
研究的目的:
- 为了证明循环食批次发酵 (CFBF) 提高了PHA生产力.
- 作为唯一的碳来源,利用来自纤维素酸废物的富含葡萄糖的水解盐.
- 为了优化发酵条件以获得高细胞密度生物质和PHA产量.
主要方法:
- 在统计学上优化了Bacillus thuringiensis的发酵条件.
- 实施一个三周期CFBF战略.
- 使用FTIR光谱和热分析分析PHA组成和特性.
主要成果:
- 在三次CFBF循环后,细胞生物质达到20.99g L-1和PHA度达到14.28g L-1.
- 获得的PHA产量为68.03%和生产率为0.219克L-1 h-1,比批量发酵显著增加.
- 在第一个循环后识别了PHA共聚合物和随后的循环后的聚合物,其热稳定性与商业PHB相比.
结论:
- 与传统批量发酵相比,CFBF显著提高了PHA生产率和生物质度.
- 纤维素废弃物水解盐可以作为一种可行的原料,用于成本效益高的PHA生物塑料生产.
- 这种方法为高密度生物质和增强PHA生产提供了一个可持续的战略.
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