小麦质水解剂在非常高的重力中促进酒酵母的发酵性能
Huirong Yang1, Teodora Emilia Coldea2, Yingjie Zeng1
1College of Food Science and Technology, Southwest Minzu University, Chengdu, 610041, China.
Bioresources and bioprocessing
|April 23, 2024
概括
小麦质水解剂 (WGH) 在极高重力 (VHG) 料中改善酒酵母发酵. 用40%的乙醇部分补充剂显著提高了酵母的性能,乙醇产量和细胞健康.
科学领域:
- 生物技术是生物技术.
- 发酵科学 发酵科学
- 酵母生理学酵母生理学
背景情况:
- 非常高重力 (VHG) 发酵对酒酵母提出了挑战,原因是透应激和营养限制.
- 在VHG条件下提高酵母性能对于高效的乙醇生产至关重要.
研究的目的:
- 为了研究小麦质水解剂 (WGH) 和它们的部分在VHG发酵中对酒酵母的影响.
- 为了确定最佳的WGH分数来增强酵母生理活动和发酵结果.
主要方法:
- 酒酵母在VHG中发酵,并补充了WGH和各种乙醇化分数.
- 测量了酵母生物量,生存能力,发酵能力,乙醇产量和生产力.
- 分析了细胞生理参数,包括氨基酸消耗,糖醇和三糖积累,细胞膜完整性,线粒体膜潜力 (MMP) 和反应性氧物种 (ROS) 水平.
主要成果:
- WGH及其分离物显著提高了酵母生物质,活力,发酵能力,乙醇产量和VHG中的生产力.
- 40%的乙醇分量产生了最高的结果:6.9 g/L干细胞生物质,82.05%的发酵率和12.19% (v/v) 的乙醇标位.
- 这部分还促进了氨基酸消耗,增加了细胞内甘油和三糖,提高了细胞膜完整性15.39%,增强了MMP39.61%,并减少了ROS18.94%.
结论:
- 小麦质水解剂是提高酒酵母在VHG发酵过程中的性能的有效补充剂.
- 40%的乙醇部分WGH显示出优化VHG发酵过程和增强酵母强度的巨大潜力.
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