对Trichoderma reesei的比较转录组分析揭示了由葡萄糖和β-二糖化物的合成混合物诱导的不同基因调节网络
Yonghao Li1, Jingze Yu2, Peng Zhang2
1Chongqing Key Laboratory of Industrial Fermentation Microorganism and School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing, 401331, China. yh_li@cqust.edu.cn.
Bioresources and bioprocessing
|April 23, 2024
概括
葡萄糖和β-二糖化物 (MGD) 的混合物通过上调关键的转录因子和通路,有效地诱导Trichoderma reesei中的细胞酶的产生. 这一发现为基纤维素生物质生物提炼提供了新的策略.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- 皮虫 (Trichoderma reesei) 对于纤维素生物质降解至关重要.
- 在T. reesei中,细胞酶的产生是由特定的碳来源诱导的.
- 葡萄糖和β-二糖化物 (MGD) 的混合物是一种潜在的低成本诱导剂.
研究的目的:
- 研究MGD诱导T. reesei.细胞酶产生的分子机制.
- 用转录组分析将MGD诱导与乳糖诱导进行比较.
- 确定参与MGD介导酶生产的关键基因和途径.
主要方法:
- 对T. reesei Rut C30.的转录组分析
- 由MGD和乳糖诱导的基因表达特征的比较.
- 专注于碳水化合物活性酶 (CAZymes),转录因子和蛋白质处理途径.
主要成果:
- 与乳糖相比,MGD显著上调细胞酶编码基因 (GH5,GH6,GH7).
- MGD下调了编码西兰酶的基因 (GH11,GH74).
- 在MGD中观察到转录因子xyr1,vib1和hac1的升调,以及基因激活蛋白激酶通路的强化.
结论:
- MGD是T. reesei.中细胞酶生产的强有力的诱导剂.
- 与乳糖不同,MGD会影响红纤维素降解酶的特征.
- 这些发现为了解MGD的作用和改善T. reesei菌株用于生物提炼提供了基础.
相关概念视频
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Global Regulatory Systems
959
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
959
Gene Regulation During Sporulation
713
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
713
Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67


