化上腺髓微生理系统以模拟尼古丁和阿片类药物暴露
Jonathan R Soucy1, Gabriel Burchett1, Ryan Brady1
1Chemical Engineering, Northeastern University, Boston, MA, USA.
Organs-on-a-chip
|April 23, 2024
概括
新生儿上腺功能,对于适应子宫外的生活至关重要,使用3D微生理系统 (MPS) 更好地理解. 这些系统揭示了同情神经元如何控制catecholamine释放,即使暴露在阿片类药物或尼古丁中.
科学领域:
- 生理学 生理学 生理学
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
背景情况:
- 过渡到子宫外生命需要一个catecholamine激增的心血管,呼吸和代谢适应.
- 上腺细胞中甲醇胺释放的机制尚未完全理解.
- 剖腹产和产前暴露于尼古丁或阿片类药物会损害上腺母细胞类甲醇胺释放.
研究的目的:
- 为了研究调节上腺膜内置的机制.
- 开发和利用一种新的生物工程模型来研究上腺髓.
主要方法:
- 使用GelPins开发分隔的3D微生理系统 (MPS).
- 使用连续微组织内的上腺色氨酸细胞和前关节交感神经元的离散培养.
- 在不同氧气条件下以及在阿片类药物和尼古丁的存在下评估catecholamine释放.
主要成果:
- 上腺克罗马芬内置对于低氧介导的甲基醇胺释放至关重要.
- 阿片类药物和尼古丁会影响上腺蛋白细胞对低氧的反应.
- 控制甲基荷氨酸释放的神经控制机制尽管暴露于阿片类药物和尼古丁,但仍然完好无损.
结论:
- 基于GelPin的MPS为研究内化器官系统提供了一个有效的平台.
- 这种模型有助于我们更好地理解catecholamine释放调节的作用.
- 该平台为改善神经内分泌研究中的药物查提供了潜力.
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