血清素对于深海菌的致病性至关重要Vibrio fluvialis
Yujian Wang1,2, Jingchang Luo1,2,3, Yan Zhao4
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China.
iScience
|April 23, 2024
概括
一种深海的Vibrio fluvialis分离物通过诱导热死引起致命的感染. 德尔塔病毒流动性血溶素 (δVFH) 对于这种毒性至关重要,它通过特定的膜脂相互作用起作用.
科学领域:
- 微生物学 微生物学
- 病原体研究 病原体研究
- 分子生物学分子生物学
背景情况:
- 流动性菌是一种新兴的食源性病原体.
- 流体血溶素 (VFH) 和三角形流体血溶素 (δVFH) 是由V. fluvialis产生的,但δVFH的功能尚不清楚.
- 此前没有报告过致病深海V. fluvialis.
研究的目的:
- 为了研究深海Vibrio fluvialis隔离物 (V13) 的致病性.
- 为了阐明 δVFH 在 V. fluvialis 毒性的作用.
- 了解V. fluvialis诱导的主体细胞死亡背后的分子机制.
主要方法:
- 在鱼类模型中对深海V. fluvialis分离物 (V13) 的致病性测试.
- 基因分析比较V13与已知的V. fluvialis菌株.
- 对V13及其VFH/δVFH突变体的细胞毒性评估.
- 对重组 δVFH 诱导的火灭菌的研究,包括离子流和脂质结合试验.
主要成果:
- 深海V. fluvialis V13分离物导致鱼类致死性感染,并通过NLRP3炎症酶,Casp1 (Casp1) 和Gasdermin D (GSDMD) 激活诱导热.
- VFH和δVFH对于V. fluvialis细胞毒性至关重要,而δVFH起着重要作用.
- 再组合 δVFH 诱导了依赖 K+ 流量和 Ca2+ 积累的灭,其细胞毒性与结合血脂相关.
结论:
- 深海的Vibrio fluvialis可能是致病的,并诱导热亡.
- 德尔塔病毒流动性血溶素 (δVFH) 是细胞毒性至关重要的关键毒性因子.
- δVFH的机制涉及血脂相互作用,K+流出和Ca2+积累,导致热.
关键词:
结构 结构 结构 结构 结构
相关概念视频
00:43Lysogenic Cycle of Bacteriophages
62.1K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.1K
01:10Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K