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在Mylabris sibirica (Coleoptera, Meloidae) 中进行定量表达分析的参考基因评估

Chen-Hui Shen¹, Min Tang¹, Xiao-Fei Li¹

¹Key Laboratory of Agricultural Genetically Modified Organisms Traceability, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Science/Supervision and Test Center (Wuhan) for Plant Ecological Environment Safety, Ministry of Agriculture and Rural Affairs, Wuhan, China.

Frontiers in physiology

|April 23, 2024

概括

这项研究确定了Mylabris sibirica.分子研究的最佳内部参考基因. 建议RPL6和RPL13用于在各种条件下进行基因表达分析,以确保对这种昆虫进行准确的研究.

科学领域:

分子生物学分子生物学
昆虫学昆虫学是一门学科.
遗传学是一个遗传学.

背景情况:

米拉布里斯·西比里卡 (Mylabris sibirica) 是一种超变态的昆虫,以油菜为食.
由于缺乏合适的内源性参考基因,分子研究的数量有限.

研究的目的:

为了评估Mylabris sibirica中的十个候选内部参考基因.
为了在不同的实验条件下确定最稳定的基因参考基因,进行精确的基因表达分析.

主要方法:

使用了实时定量PCR (RT-qPCR).
五种统计算法 (Ct值,geNorm,NormFinder,BestKeeper,RefFinder) 用于评估. 这种算法被用于评估.
在四种治疗方法中分析了基因基因稳定性:成人年龄,成人组织,温度和性别.

主要成果:

RPL6和RPL13被确定为成人年龄和温度最稳定的组合.
建议RPL6和RPS18用于不同的成年组织.
发现AK和RPL6分别是男性和女性成年人的最佳基因参考基因.

结论:

RPL6和RPL13是M. sibirica在各种疾病中的基因表达研究中最合适的参考基因对.
准确的正常化至关重要,正如使用不同的参考基因时MsUAP的可变转录水平所证明的那样.
这项研究为未来对M. sibirica.分子生理学和生物化学研究提供了基础.

关键词:

这就是Mylabris sibirica.RT-qPCRR 是一个人.在RefFinder中找到.在GeNorm上使用.基因参考基因是指基因的参考基因.

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