在韩国儿童中对抗塞夫特里亚克松的海莫菲勒斯流感的表征
Sanghoon Lee1, Gahee Kim1, Jung Hwa Kim1
1Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.
Journal of Korean medical science
|April 23, 2024
概括
在儿童中发现了抗塞夫特里亚克森的Haemophilus influenzae菌株,主要与PBP3变异 (如S385T和L389F) 相关. 仅仅N526K突变对耐药性的影响很小.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 遗传学 是一个遗传学.
背景情况:
- 血型流感是儿童呼吸道感染的常见原因之一.
- 检测出抗塞夫的抗菌株促使人们对抗药性机制进行了调查.
- 专注于青素结合蛋白3 (PBP3) 的改变和β-乳糖酶的产生.
研究的目的:
- 在H.中研究 ceftriaxone耐药性的机制. 流感是一种流感.
- 确定与耐药性相关的特定PBP3突变和β-乳糖酶存在.
- 确定这些机制对凯夫特里亚克森最小抑制度 (MIC) 的影响.
主要方法:
- 对抗 ceftriaxone 的 H. 的分析. 来自儿童医院 (2014-2019) 的流感分离物 (2014-2019).
- 使用E测试 (EUCAST指南) 确定塞夫特里亚克森MIC.
- 通过Cefinase测试和PCR检测β-乳酸酶;通过ftsI基因测序分析PBP3变异.
主要成果:
- 分析了19个抗塞夫特里亚的分离物;18个是非类型的H. 流感是一种流感.
- 12个分离物对TEM-1 PCR检测呈阳性;确定了各种PBP3变异模式 (II,III,III+,III类+组).
- 在III类+ (S385T+L389F+R517H) 和III类+ (S385T+L389F+N526K) 组中, ceftriaxone MIC的中位数最高.
结论:
- 在儿科中对塞夫特里亚克森的耐药性H. 韩国正在出现流感,与S385T和L389F的PBP3替代物有关.
- 单独的N526K突变并没有显著地导致对 ceftriaxone 的耐药性.
- 需要进一步进行大规模研究,以监测儿科H的抗生素耐药性趋势. 流感是一种流感.
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