乳腺癌患者外周血液中的自然杀手细胞子集及其功能分子
Reza Darvishvand1,2, Somayeh Rezaeifard2, Razie Kiani2
1Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Immunity, inflammation and disease
|April 23, 2024
概括
乳腺癌患者的自然杀手 (NK) 细胞显示功能分子发生变化,CXCR3增加,但大酶B减少,表明潜在的免疫功能障碍. 这些发现突出了乳腺癌进展期间NK细胞表型的变化.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 自然杀手 (NK) 细胞对于癌症免疫监测至关重要.
- 这项研究研究了乳腺癌患者的传统和新型NK细胞子集.
- 在乳腺癌患者和健康捐赠者之间比较NK细胞的功能分子.
研究的目的:
- 在乳腺癌中评估传统的 (CD56/CD16) 和新的 (CD56/CD27/CD11b) NK 细胞子集.
- 为了比较乳腺癌患者和健康个体之间NK细胞中功能分子的表达.
- 评估观察到的NK细胞改变对乳腺癌发病的潜在影响.
主要方法:
- 流细胞计用于分析30名乳腺癌患者和20名健康捐赠者的外周血液NK细胞.
- 针对CD3,CD56,CD16,CD27,CD11b,CD96,NKG2C,NKG2D,NKp44,CXCR3,孔素和B粒酶的抗体被使用.
- 测量了各种NK细胞标记物和功能分子的平均光强度 (MFI).
主要成果:
- 乳腺癌患者和健康捐赠者之间没有观察到总NK细胞或子集的频率有显著差异.
- 在乳腺癌患者的总NK细胞和常规细胞毒性NK细胞 (CD3-CD56dimCD16+) 中发现CXCR3的MFI升高.
- 在乳腺癌患者的常规调节NK细胞 (CD3-CD56brightCD16-/+) 和CD3-CD56-CD16+NK细胞中观察到较低的granzyme B MFI.
结论:
- 增加NK细胞上的CXCR3表达可能会增加它们透到乳腺癌组织中.
- 减少的B粒酶表达表明乳腺癌患者NK细胞的细胞毒性活性降低.
- 这些发现表明,乳腺癌患者的外周血液中可能存在功能障碍的NK细胞子集的积累.
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