相关实验视频

Updated: Jun 28, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

从白植物Phylloglossum drummondii对U-to-CRNA编辑的洞察力

Farley M Kwok van der Giezen¹, Amy Viljoen¹, Leni Campbell-Clause¹

¹ARC Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.

The Plant journal : for cell and molecular biology

|April 23, 2024

概括

普罗格洛苏姆·德鲁蒙迪 (Phylloglossum drummondii) 呈现出罕见的尿素对细胞氨基酸RNA编辑. 研究人员确定了四个保存的线粒体U-to-CRNA编辑部位和白细胞中的潜在酶,为这个鲜为人知的机制提供了洞察力.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
RNA生物学的RNA生物学

背景情况:

百合植物Phylloglossum drummondii在其属中是独一无二的,并表现出尿素对细胞蛋白RNA编辑.
器官细胞RNA编辑是植物中一个关键的转录后修改.

研究的目的:

组装和分析P.drummondii.的叶绿体和线粒体基因组.
综合描述器官RNA编辑事件,重点是U-to-C编辑.
在相关的白细胞中识别潜在的U-to-CRNA编辑酶.

主要方法:

基因组组合P. drummondii的叶绿体和线粒体.
RNA测序以识别编辑站点.
对于相关的白植物来说,de novo转录组组合.
生物信息分析以确定编辑因素.

主要成果:

聚合了P. drummondii的 хлоропласт和线粒体基因组.
在线粒体转录 (cob, nad1, nad5, rpl2) 中识别了众多C-to-U编辑事件和四个U-to-C编辑事件.
在相关的白植物 (Huperzia,Phlegmariurus) 中保存U-到C编辑部位.
识别了与观察到的编辑部位相对应的四个假定的U-to-CRNA编辑因子.

结论:

P. drummondii 和相关的白植物具有有限但保存的线粒体U-to-CRNA编辑事件.
已识别的编辑因素为了解U-to-CRNA编辑的机制提供了基础.
这些白植物作为研究不太了解的U-to-CRNA编辑途径的有价值的模型.

关键词:

菲洛格洛斯斯姆 (英语:Phylloglossum) 是一个名字.хлоропласт 是一种质塑料.这些PPR蛋白质是PPR蛋白质.编辑RNA的RNA编辑线粒体中的线粒体.

更多相关视频

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Bacterial Transcription

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.